There will be a HazMat fee per item when shipping a dangerous goods. The HazMat fee will be charged to your UPS/DHL/FedEx collect account or added to the invoice unless the package is shipped via Ground service. Ship by air in Excepted Quantity (each bottle), which is up to 1g/1mL for class 6.1 packing group I or II, and up to 25g/25ml for all other HazMat items.
Type
HazMat fee for 500 gram (Estimated)
Excepted Quantity
USD 0.00
Limited Quantity
USD 15-60
Inaccessible (Haz class 6.1), Domestic
USD 80+
Inaccessible (Haz class 6.1), International
USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic
USD 100+
Accessible (Haz class 3, 4, 5 or 8), International
The family of HIF-PH (HIF prolyl hydroxylase) enzymes are the main molecular to regulate HIF activity through hydroxylation at 2 proline residues and cause the degradation of HIF, thus leading to inhibition on accumulation of functional HIF under normoxia. DMOG (Dimethyloxalylglycine) is a prolyl 4-hydroxylase inhibitor with IC50 value of 2 μM (measured by hydroxyproline formation in chicken embryo calvaria) [1]. HIF-1α is strongly induced in Hep3B and U2OS cells exposure to 100 μM or 1000 μM DMOG for 6h under normoxic culture conditions[2]. Administration to newborn pups by i.p. injections at a dose of 200 mg/kg DMOG conferred a protective phenotype in the retinopathy of prematurity (ROP) model[3].
作用机制
DMOG, an analog to 2OG, can inhibit prolyl 4-hydroxylase in a competitive manner[4]. It is the most structurally basic 2OG-derived HIF stabilizer[3].
DMOG/二甲基草酰甘氨酸 细胞实验
Cell Line
Concentration
Treated Time
Description
References
JJ012 cells
0.5 mM
48 h
Stabilize HIF1α protein and prevent its degradation, results showed elevated protein levels of HIF1α and total PYGL
Cell Rep. 2023 Jun 27;42(6):112578.
HT1080 cells
1 mM
24 h
Stabilize HIF1α protein and prevent its degradation, results showed HIF1α interacts with mutant IDH1 in the nuclear fraction
Cell Rep. 2023 Jun 27;42(6):112578.
DMOG/二甲基草酰甘氨酸 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Trypanosoma brucei
H3.VKO cell line
Media supplementation
1mM
5 days
DMOG treatment resulted in the complete loss of base J but no termination defects were found
PLoS Genet. 2016 Jan 21;12(1):e1005758
Mice
Antibiotic-associated CDI model
Intraperitoneal injection
400 mg/kg
Starting on the day of C. difficile gavage, lasting for 2 days
To study the effect of DMOG on CDI mice, results showed that DMOG significantly exacerbated the death rate in CDI mice
Nat Microbiol. 2019 Feb;4(2):269-279.
Mice
Wild-type mice
Intraperitoneal injection
40 mg/kg
Once daily for 3 days
DMOG-treated mice exhibited cold intolerance with attenuated thermogenic gene expression and beige adipocyte formation in iWAT upon cold exposure
Nat Commun. 2022 Jun 7;13(1):3268
Mice
Wild-type mice
Intraperitoneal injection
40 mg/kg
Daily for 3 days
DMOG-administered WT mice were cold intolerant with attenuated thermogenic gene expression and beige adipocyte formation in iWAT upon cold.
Nat Commun. 2022 Jun 7;13(1):3268
Mice
Pulmonary fibrosis model
Intraperitoneal injection
50 mg/kg
2, 5, 8, 11 days
DMOG reversed the inhibitory effect of AsA on HIF-1α, increasing tumor volume, mediastinal lymph node weight, metastatic lung nodule count, and hydroxyproline levels.
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