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/ 5-BrdU/5-溴-2'-脱氧尿苷

5-BrdU/5-溴-2'-脱氧尿苷 {[allProObj[0].p_purity_real_show]}

货号：A386942 同义名： 5-溴脱氧尿嘧啶核苷 / BrdU; 5-Bromo-2'-deoxyuridine

5-BrdU（BrdU）是一种核苷类似物，能与胸苷竞争插入DNA中，常用于检测增殖细胞。

5-BrdU/5-溴-2'-脱氧尿苷 化学结构 CAS号：59-14-3
5-BrdU/5-溴-2'-脱氧尿苷 化学结构
CAS号：59-14-3
5-BrdU/5-溴-2'-脱氧尿苷 3D分子结构
CAS号：59-14-3
5-BrdU/5-溴-2'-脱氧尿苷 化学结构 CAS号：59-14-3
5-BrdU/5-溴-2'-脱氧尿苷 3D分子结构 CAS号：59-14-3
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5-BrdU/5-溴-2'-脱氧尿苷 纯度/质量文件 产品仅供科研

货号：A386942 标准纯度： {[allProObj[0].p_purity_real_show]}
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产品名称 DNA synthesis ↓ helicase ↓ RdRp ↓ ribonucleotide reductase ↓ tRNA synthetase ↓ YB-1 ↓ 其他靶点 纯度
Fexinidazole ✔ 98%
Daptomycin ✔ 98%
Blasticidin S·HCl ✔ 98%
Metronidazole ✔ 98%
Daunorubicin HCl +++

DNA synthesis, Ki: 20 nM

 		98%
Triglycidyl isocyanurate ✔ p53 98+%
Nedaplatin ✔ 99%+
Oxolinic acid ✔ 98+%
Bendamustine ✔ 98+%
Trifluridine ✔ 98%
Robinetin ✔ 99%+
Carboplatin ✔ 99%
Cidofovir ✔ 99%
Cisplatin ✔ 99%
Cytarabine ++++

DNA synthesis, IC50: 16 nM

 		98%
Acelarin ++++

DNA synthesis, EC50: 0.2 nM

 		99%+
Oxaliplatin ✔ 98%
YK-4-279 ✔ 99%+
ML216 +

BLM636-1298, IC50: 0.97 μM

BLMfull-length, IC50: 2.98 μM

 		99%+
RK-33 ✔ 98%
Brr2-IN-3 ✔ 99%+
Phen-DC3 Trifluoromethanesulfonate ✔ 95%
Favipiravir ✔ 99%
Suramin sodium salt ++

RdRp, IC50: 0.26 μM

 		99%+
Clofarabine ++

Ribonucleotide reductase, IC50: 65 nM

 		97%
Didox ✔ 98%
(E)-3-AP ✔ 99%
Halofuginone +++

prolyl-tRNA synthetase, Ki: 18.3nM

 		99%+
BC-LI-0186 +++

Leucyl-tRNA synthetase, Kd: 42.1 nM

Leucyl-tRNA synthetase, IC50: 46.11 nM

 		98%
SU056 +

YB-1, IC50: 1.73 μM

 		98%
1. 鼠标悬停在“+”上可以显示相关IC50的具体数值。"+"越多，抑制作用越强。2. "✔"表示该化合物对相应的亚型有抑制作用，但抑制强度暂时没有相关数据。

5-BrdU/5-溴-2'-脱氧尿苷 生物活性

描述 Bromodeoxyuridine (BrdUrd), a thymidine analogue incorporated into DNA, can be quantified by fluorescent or chromophoric quenching of dyes bound to DNA or with antibodies to BrdUrd[3]. Bromodeoxyuridine, even at a level of substitution into newly mad DNA of 95%, has no effect on the 5-methylcytosine content of DNA. At all levels of bromodeoxyuridine substitution, highly repetitive DNA has slightly more 5-methylcytosine (3.0% of total cytosine) than does single copy DNA or moderately repetitive DNA (2.3%)[4]. A single, brief in vitro exposure to BrdU induces a profound and sustained reduction in the proliferation rate of all cancer cells examined. Cells do not die but variably up-regulate some senescence-associated proteins as they accumulate in the G1 phase of the cell cycle. Bromodeoxyuridine also impairs the proliferative capacity of primary tumor-initiating human glioma cells and may therefore represent a means of targeting cancer stem cells[5]. Bromodeoxyuridine (BrdU) is widely used in immunology to detect cell division, and several mathematical models have been proposed to estimate proliferation and death rates of lymphocytes from BrdU labelling and de-labelling curves[6]. The BrdU-dependency of arrest in the G2 phase can be used as a sensitive cell biological assay to detect DNA damage elicited by oxygen free radicals[7].

5-BrdU/5-溴-2'-脱氧尿苷 临床研究

NCT号 适应症或疾病 临床期 招募状态 预计完成时间 地点
NCT03371134 - Not yet recruiting July 26, 2020 -
NCT00003405 Leukemia Phase 2 Unknown - United States, Illinois ... 展开 >> Cook County Hospital Chicago, Illinois, United States, 60612-9985 Rush Cancer Institute Chicago, Illinois, United States, 60612 Angelo P. Creticos, M.D. Cancer Center Chicago, Illinois, United States, 60657 Rush-Riverside Cancer Center Kankakee, Illinois, United States, 60901 收起 <<
NCT02576145 - Completed - -

5-BrdU/5-溴-2'-脱氧尿苷 参考文献

[1]Levkoff LH, Marshall GP 2nd, et al. Bromodeoxyuridine inhibits cancer cell proliferation in vitro and in vivo. Neoplasia. 2008 Aug;10(8):804-16.

[2]Rothaeusler K, Baumgarth N. Assessment of cell proliferation by 5-bromodeoxyuridine (BrdU) labeling for multicolor flow cytometry. Curr Protoc Cytom. 2007 Apr;Chapter 7:Unit7.31.

[3]Dolbeare F. Bromodeoxyuridine: a diagnostic tool in biology and medicine, Part I: Historical perspectives, histochemical methods and cell kinetics. Histochem J. 1995 May;27(5):339-69

[4]Singer J, Stellwagen RH, Roberts-Ems J, Riggs AD. 5-Methylcytosine content of rat hepatoma DNA substituted with bromodeoxyuridine. J Biol Chem. 1977 Aug 10;252(15):5509-13

[5]Levkoff LH, Marshall GP 2nd, Ross HH, Caldeira M, Reynolds BA, Cakiroglu M, Mariani CL, Streit WJ, Laywell ED. Bromodeoxyuridine inhibits cancer cell proliferation in vitro and in vivo. Neoplasia. 2008 Aug;10(8):804-16

[6]Ganusov VV, De Boer RJ. A mechanistic model for bromodeoxyuridine dilution naturally explains labelling data of self-renewing T cell populations. J R Soc Interface. 2013 Jan 6;10(78):20120617

[7]Poot M, Rabinovitch PS, Hoehn H. Bromodeoxyuridine amplifies free-radical-mediated DNA damage. Biochem J. 1989 Jul 1;261(1):269-71

5-BrdU/5-溴-2'-脱氧尿苷 实验方案

计算器
存储液制备 1mg 5mg 10mg

1 mM

5 mM

10 mM

3.26mL

0.65mL

0.33mL

16.28mL

3.26mL

1.63mL

32.56mL

6.51mL

3.26mL

5-BrdU/5-溴-2'-脱氧尿苷 技术信息

CAS号59-14-3
分子式C9H11BrN2O5
分子量 307.1
SMILES Code OC[C@@H]1[C@H](C[C@H](N2C(NC(C(Br)=C2)=O)=O)O1)O
MDL No. MFCD00006529
别名 5-溴脱氧尿嘧啶核苷 ;BrdU; 5-Bromo-2'-deoxyuridine; NSC 38297; Broxuridine; Bromodeoxyuridine; BUdR
运输蓝冰
InChI Key WOVKYSAHUYNSMH-RRKCRQDMSA-N
Pubchem ID 6035
存储条件

In solvent -20°C:3-6个月-80°C:12个月

Pure form Keep in dark place, inert atmosphere, store in freezer, under -20°C
溶解方案

DMSO: 250 mg/mL(814.07 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

H2O: 2 mg/mL(6.51 mM)，配合低频超声，并水浴加热至45℃助溶

请根据您的动物给药指南选择适当的溶解方案。
以下溶解方案都请先按照体外实验的方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议现用现配，当天使用； 以下溶剂前显示的百分比是指该溶剂在终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶
方案一
方案二
配制的工作液建议现用现配，短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。
方案一

Tags: 5-BrdU | BrdU;5-Bromo-2'-deoxyuridine;NSC 38297;Broxuridine;Bromodeoxyuridine;BUdR | 5-溴脱氧尿嘧啶核苷 | Nucleoside Antimetabolite/Analog | DNA/RNA Synthesis | AmBeed-信号通路专用抑制剂 | 5-BrdU/5-溴-2'-脱氧尿苷 纯度/质量文件 | 5-BrdU/5-溴-2'-脱氧尿苷 亚型比较 | 5-BrdU/5-溴-2'-脱氧尿苷 生物活性 | 5-BrdU/5-溴-2'-脱氧尿苷 临床数据 | 5-BrdU/5-溴-2'-脱氧尿苷 参考文献 | 5-BrdU/5-溴-2'-脱氧尿苷 实验方案 | 5-BrdU/5-溴-2'-脱氧尿苷 技术信息

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