5-BrdU

产品说明书

Print
Chemical Structure| 59-14-3 同义名 : 5-溴脱氧尿嘧啶核苷 ;BrdU; 5-Bromo-2'-deoxyuridine; NSC 38297; Broxuridine; Bromodeoxyuridine; BUdR
CAS号 : 59-14-3
货号 : A386942
分子式 : C9H11BrN2O5
纯度 : 98%
分子量 : 307.1
MDL号 : MFCD00006529
存储条件：

Pure form Keep in dark place, inert atmosphere, store in freezer, under -20°C

In solvent -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 250 mg/mL(814.07 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

H2O: 2 mg/mL(6.51 mM)，配合低频超声，并水浴加热至45℃助溶
动物实验配方：
生物活性
描述 Bromodeoxyuridine (BrdUrd), a thymidine analogue incorporated into DNA, can be quantified by fluorescent or chromophoric quenching of dyes bound to DNA or with antibodies to BrdUrd[3]. Bromodeoxyuridine, even at a level of substitution into newly mad DNA of 95%, has no effect on the 5-methylcytosine content of DNA. At all levels of bromodeoxyuridine substitution, highly repetitive DNA has slightly more 5-methylcytosine (3.0% of total cytosine) than does single copy DNA or moderately repetitive DNA (2.3%)[4]. A single, brief in vitro exposure to BrdU induces a profound and sustained reduction in the proliferation rate of all cancer cells examined. Cells do not die but variably up-regulate some senescence-associated proteins as they accumulate in the G1 phase of the cell cycle. Bromodeoxyuridine also impairs the proliferative capacity of primary tumor-initiating human glioma cells and may therefore represent a means of targeting cancer stem cells[5]. Bromodeoxyuridine (BrdU) is widely used in immunology to detect cell division, and several mathematical models have been proposed to estimate proliferation and death rates of lymphocytes from BrdU labelling and de-labelling curves[6]. The BrdU-dependency of arrest in the G2 phase can be used as a sensitive cell biological assay to detect DNA damage elicited by oxygen free radicals[7].
临床研究
NCT号 适应症或疾病 临床期 招募状态 预计完成时间 地点
NCT03371134 - Not yet recruiting July 26, 2020 -
NCT00003405 Leukemia Phase 2 Unknown - United States, Illinois ... 展开 >> Cook County Hospital Chicago, Illinois, United States, 60612-9985 Rush Cancer Institute Chicago, Illinois, United States, 60612 Angelo P. Creticos, M.D. Cancer Center Chicago, Illinois, United States, 60657 Rush-Riverside Cancer Center Kankakee, Illinois, United States, 60901 收起 <<
NCT02576145 - Completed - -
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

3.26mL

0.65mL

0.33mL

16.28mL

3.26mL

1.63mL

32.56mL

6.51mL

3.26mL
参考文献

[1]Levkoff LH, Marshall GP 2nd, et al. Bromodeoxyuridine inhibits cancer cell proliferation in vitro and in vivo. Neoplasia. 2008 Aug;10(8):804-16.

[2]Rothaeusler K, Baumgarth N. Assessment of cell proliferation by 5-bromodeoxyuridine (BrdU) labeling for multicolor flow cytometry. Curr Protoc Cytom. 2007 Apr;Chapter 7:Unit7.31.

[3]Dolbeare F. Bromodeoxyuridine: a diagnostic tool in biology and medicine, Part I: Historical perspectives, histochemical methods and cell kinetics. Histochem J. 1995 May;27(5):339-69

[4]Singer J, Stellwagen RH, Roberts-Ems J, Riggs AD. 5-Methylcytosine content of rat hepatoma DNA substituted with bromodeoxyuridine. J Biol Chem. 1977 Aug 10;252(15):5509-13

[5]Levkoff LH, Marshall GP 2nd, Ross HH, Caldeira M, Reynolds BA, Cakiroglu M, Mariani CL, Streit WJ, Laywell ED. Bromodeoxyuridine inhibits cancer cell proliferation in vitro and in vivo. Neoplasia. 2008 Aug;10(8):804-16

[6]Ganusov VV, De Boer RJ. A mechanistic model for bromodeoxyuridine dilution naturally explains labelling data of self-renewing T cell populations. J R Soc Interface. 2013 Jan 6;10(78):20120617

[7]Poot M, Rabinovitch PS, Hoehn H. Bromodeoxyuridine amplifies free-radical-mediated DNA damage. Biochem J. 1989 Jul 1;261(1):269-71