Ursolic acid, a naturally occurring triterpenoid, induces the apoptosis of human cancer cells through multiple signaling pathways. A 50 µmol/l dose of ursonic acid decreased the mRNA expression levels of anti-apoptotic NF-κBp65 and Bcl-2 0.17 (8.9/52.6)-fold and 0.22 (9.5/42.3)‑fold, respectively. The proliferative activity of T24 cells treated with 12.5, 25.0 and 50.0 µmol/l ursolic acid was significantly reduced compared with that of control cells (83.8, 56.2 and 31.5 vs. 97.6%, respectively, P<0.05 for each). Ursolic acid is important in inducing apoptosis via the suppression of Akt/NF-κB signaling in T24 human bladder cancer cells and this occurs in a dose-dependent manner[3]. Treatment of B16F-10 cells with nontoxic concentration of ursolic acid showed the presence of apoptotic bodies and induced DNA fragmentation in a dose depended manner. The pro-inflammatory cytokine production and gene expression of TNF-alpha, IL-1beta, IL-6 and GM-CSF were down regulated in ursolic acid treated cells compared to nontreated B16F-10 metastatic melanoma cells[4]. UA (Ursonic Acid) inhibited constitutive and TNF-α-induced activation of NF-κB in DU145 and LNCaP cells in a dose-dependent manner. Furthermore, UA suppressed both constitutive and inducible STAT3 activation in prostate cancer cells concomitant with suppression of activation of upstream kinases (Src and JAK2) and STAT3-dependent reporter gene activity. In vivo, UA (200 mg/kg b.w.) treated for 6 weeks inhibited the growth of DU145 cells in nude mice without any significant effect on body weight[5].
Ursonic acid/熊果酮酸 细胞实验
Cell Line
Concentration
Treated Time
Description
References
Vero cells
2.5, 5, 10 µM
16 hours
To evaluate the antiviral effect of UNA on PEDV. The results showed that UNA significantly inhibited PEDV replication.
Vet Res. 2024 May 23;55(1):67
ST cells
2.5, 5, 10 µM
18 hours
To evaluate the antiviral effect of UNA on SVA. The results showed that UNA significantly inhibited SVA replication.
Vet Res. 2024 May 23;55(1):67
Porcine alveolar macrophages (PAMs)
2.5, 5, 10 µM
24 hours
To verify the antiviral activity of UNA in porcine cells. The results showed that UNA significantly decreased the PRRSV ORF7 mRNA level, N protein expression, and viral titre in a concentration-dependent manner.
Vet Res. 2024 May 23;55(1):67
Marc-145 cells
2.5, 5, 10 µM
48 hours
To evaluate the inhibitory effect of UNA on PRRSV replication. The results showed that UNA inhibited PRRSV genome replication, viral protein expression and virus production in a dose-dependent manner.
Vet Res. 2024 May 23;55(1):67
Ursonic acid/熊果酮酸 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
C57BL/6 mice
Experimental autoimmune encephalomyelitis (EAE) model
Oral
50 mg/kg
Once daily for 10 days
To evaluate the therapeutic effects of ursonic acid on multiple sclerosis, showing significant improvement in clinical symptoms and pathological features of the EAE model.
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