Sennoside B can inhibit PDGF-stimulated cell proliferation by binding to PDGF(platelet-derived growth factor)-BB and its receptor and by down-regulating the PDGFR-beta signaling pathway[3]. The 50% inhibitory concentrations of the four sennosides (A, B, E and F) were 17, 9, 24 and 13 microM, respectively, and the type of inhibition by seenosides A and B with respect to allylamine as the substrate was non-competitive[4]. Intracaecal administration of rhein anthrone, the intraluminally active metabolite of sennosides A and B, to mice quickly induced severe diarrhoea[5]. From acute studies, sennosides could be classified as only slightly toxic in rats and mice after a single oral dose. The LD50 values were about 5,000 mg/kg in both species. In subacute studies with rats (max. 20 mg/kg) and dogs (max. 500 mg/kg), sennosides caused no specific local or systemic toxicity[6].
Sennoside B/番泻苷 B 细胞实验
Cell Line
Concentration
Treated Time
Description
References
C8166 cells
C8166 cells
To evaluate the inhibitory activity of SA and SB against HIV-1-induced cytopathic effects (CPE). Results showed an IC50 of 4.73 μM for SB.
Front Microbiol. 2023 Sep 25;14:1270258.
L929 cells
L929 cells
To evaluate the inhibitory effect of Sennoside B on TNF-α-induced L929 cell toxicity, results showed 80.3% inhibition at 100 µM concentration
Biomedicines. 2021 Sep 17;9(9):1250.
HeLa cells
HeLa cells
To evaluate the inhibitory effect of Sennoside B on TNF-α-induced HeLa cell toxicity, results showed an IC50 value of 0.32 µM
Biomedicines. 2021 Sep 17;9(9):1250.
AGS gastric cells
AGS gastric cells
To evaluate the effect of Sennoside B on PGE2 production, results showed that Sennoside B increased PGE2 concentration in a dose-dependent manner.
Biomol Ther (Seoul). 2015 Sep;23(5):458-64.
Sennoside B/番泻苷 B 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Mice
C57BL/6 mice
Intraperitoneal injection
9.3 mg/kg
Single injection, followed by injection of Ag-treated B cells after 2 hours
To evaluate the inhibitory effect of Sennoside B on antigen transfer between B cells and macrophages/dendritic cells in vivo, results showed that Sennoside B significantly reduced antigen transfer, comparable to SR-A-deficient mice.
J Biol Chem. 2012 Feb 17;287(8):5310-6
Sprague-Dawley rats
HCl•EtOH-induced gastritis and indomethacin-induced gastric ulcer models
Oral
100 mg/kg
Single dose, 30 minutes before inducing gastritis or gastric ulcer
To evaluate the gastroprotective effects of Sennoside B, results showed that Sennoside B significantly reduced lesion indices, inhibited gastric acid secretion, and increased gastric juice pH.
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