There will be a HazMat fee per item when shipping a dangerous goods. The HazMat fee will be charged to your UPS/DHL/FedEx collect account or added to the invoice unless the package is shipped via Ground service. Ship by air in Excepted Quantity (each bottle), which is up to 1g/1mL for class 6.1 packing group I or II, and up to 25g/25ml for all other HazMat items.
Type
HazMat fee for 500 gram (Estimated)
Excepted Quantity
USD 0.00
Limited Quantity
USD 15-60
Inaccessible (Haz class 6.1), Domestic
USD 80+
Inaccessible (Haz class 6.1), International
USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic
USD 100+
Accessible (Haz class 3, 4, 5 or 8), International
Pyr3 selectively and directly inhibits TRPC3 channels as follows: Pyr3 inhibits TRPC3-mediated Ca2+ influx in a dose-dependent manner with an IC50 value of 700 nM. The effect of Pyr3 becomes evident at 0.3 μM, while TRPC3 is almost completely inhibited at 3 μM. Interestingly, Pyr3 inhibited Ca2+ influx in cells expressing both TRPC3 and TRPC6, but not in cells expressing both TRPC1 and TRPC5. Pyr3 inhibited Ang II-induced NFAT translocation in a concentration-dependent manner with an IC50 value of 0.05 μM[1].
Pyr3 细胞实验
Cell Line
Concentration
Treated Time
Description
References
MDA-MB-231
1.0 µM
120 hours
Blocking TRPC3 attenuated the proliferation of MDA-MB-231 cells and increased the percentage of cells in the sub-G1 phase.
Cancers (Basel). 2019 Apr 18;11(4):558
Submandibular gland acini
3 µM
30 min
Pyr3 inhibited carbachol-induced autophagy and mistargeting of secretory granules.
TRPC3 inhibition induced a dose-dependent reduction in cell proliferation, while cell adhesion remained unaffected.
Front Cell Dev Biol. 2024 Feb 15;12:1337714
MDA-MB-231
0.5-1.0 µM
72 hours
Blocking TRPC3 decreased the percentage of viable MDA-MB-231 cells in a concentration-dependent manner.
Cancers (Basel). 2019 Apr 18;11(4):558
Mouse mesenteric artery endothelial cells
1 μM
To validate the effect of Pyr3 on mouse mesenteric artery endothelial cells. Pyr3 significantly reduced the ACh-induced hyperpolarization amplitude.
Cardiovasc Res. 2012 Sep 1;95(4):439-47.
Rat mesenteric artery endothelial cells
1 μM
To investigate the role of TRPC3 channels in rat mesenteric artery endothelial cells. Pyr3 significantly reduced the ACh-induced hyperpolarization amplitude.
Cardiovasc Res. 2012 Sep 1;95(4):439-47.
Pyr3 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Mice
Acute pancreatitis model
Intraperitoneal injection
0.1 μg/g
Hourly for 4 times
Pyr3 reduced plasma amylase content by about 50% and alleviated pancreatic edema and pathological changes.
To evaluate the effect of Pyr3 on endothelium-dependent hyperpolarization-mediated vasodilation in TRPC3 knockout mice. Results showed Pyr3 significantly attenuated ATP-stimulated endothelium-dependent hyperpolarization-mediated vasodilation.
J Am Heart Assoc. 2014 Aug 20;3(4):e000913
Mice
Hypertensive model (BPH mice) and normotensive control (BPN mice)
Bath application
10 μM
Not specified
To investigate the vasodilatory effects of Pyr3 and Pyr10 on mesenteric arteries from BPN and BPH mice, finding that BPN arteries showed a larger vasodilatory response to Pyr3 and Pyr10.
J Physiol. 2017 Mar 1;595(5):1497-1513
Mice
XY mouse gonads
Ex vivo culture
3 µM
24-hour treatment followed by 24 and 48 hours of culture in fresh media
Pyr3-treated XY gonads exhibited a substantial reduction in germ cell numbers (2.9-fold) when compared to control XY gonads, caused by reduced germ cell proliferation. Additionally, Pyr3-treated XY gonads showed a loss of the coelomic blood vessel due to increased apoptosis of endothelial cells after 72 hours of culture.
Front Cell Dev Biol. 2024 Feb 15;12:1337714
Mice
Pilocarpine-induced Status Epilepticus model
Intraperitoneal injection
3 mg/kg
Single dose
Pyr3 selectively inhibits TRPC3 channels, significantly reducing the overall RMS power of pilocarpine-induced SE and selectively attenuating theta activity during SE.
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