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Prostaglandin E2/前列腺素E2 {[allProObj[0].p_purity_real_show]}

货号:A695566 同义名: 地诺前列酮 / PGE2; Dinoprostone

Prostaglandin E2是一种天然前列腺素,通过激活前列腺素E2受体作为血管扩张剂发挥作用。Prostaglandin E2促进来自小鼠、猕猴和人类胚胎干细胞向造血前体细胞的分化,促进来自造血前体细胞的髓源抑制细胞的分化,促进来自初始T细胞的Th17细胞的分化。

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Type HazMat fee for 500 gram (Estimated)
Excepted Quantity USD 0.00
Limited Quantity USD 15-60
Inaccessible (Haz class 6.1), Domestic USD 80+
Inaccessible (Haz class 6.1), International USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic USD 100+
Accessible (Haz class 3, 4, 5 or 8), International USD 200+
Prostaglandin E2/前列腺素E2 化学结构 CAS号:363-24-6
Prostaglandin E2/前列腺素E2 化学结构
CAS号:363-24-6
Prostaglandin E2/前列腺素E2 3D分子结构
CAS号:363-24-6
Prostaglandin E2/前列腺素E2 化学结构 CAS号:363-24-6
Prostaglandin E2/前列腺素E2 3D分子结构 CAS号:363-24-6
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Prostaglandin E2/前列腺素E2 纯度/质量文件 产品仅供科研

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Prostaglandin E2/前列腺素E2 生物活性

描述 Prostaglandin E2 (PGE2) is a potent inflammatory mediator that is generated by cyclooxygenase 2 (COX2) conversion of arachidonic acid. PGE2 was shown to stimulate progenitor proliferation in vitro in cell culture and intravenous injection of PGE on injured mice showed significant improvement in marrow recovery versus control animals[3]. PGE2 (0.1 mg/min, i.a.) increases renal blood flow. PGE2 produces a biphasic change in renal vascular resistance, vasodilatation starts at 0.01 mg/min and is maximal at about 3 mg/min, while at the highest dose used (20 mg/min) PGE2 induces renal vasoconstriction[4].

Prostaglandin E2/前列腺素E2 细胞实验

Cell Line
Concentration Treated Time Description References
Adult mouse ventricular cardiomyocytes 10−6 M 10 minutes PGE2 reduced contractility in adult mouse ventricular cardiomyocytes, as evidenced by decreased peak height, departure velocity, and return velocity. Circ Heart Fail. 2016 Aug;9(8):10.1161/CIRCHEARTFAILURE.116.003291 e003291.
Mouse jejunal epithelial stem cell spheroids 0-1000 nM 24 hours Treatment with dmPGE2 stimulated WAE cell formation, with a dose-dependent effect on spheroid size, cell flattening, and mRNA expression of the WAE cell marker Claudin-4 (Cldn4). EMBO J. 2017 Jan 4;36(1):5-24.
Human ileal epithelial stem cell spheroids 1 µM 24 hours Treatment with dmPGE2 induced morphological changes and CLDN4 mRNA expression in human ileal spheroids, and EP4 inhibitor blocked these responses. EMBO J. 2017 Jan 4;36(1):5-24.
Human ovarian microvascular endothelial cells (hOMECs) 1 µM 24 hours PGE2 enhanced migration and sprout formation but did not alter hOMEC proliferation. Hum Reprod. 2016 Feb;31(2):436-44.
Human umbilical vein endothelial cells (HUVECs) 1 µM 24 hours PGE2 reduced the number of proliferating HUVECs and did not alter their migration or sprout formation. Hum Reprod. 2016 Feb;31(2):436-44.
Murine bone marrow-derived dendritic cells 1.25-10 µg/ml 24 hours To investigate the regulatory effect of PGE2 on the migratory ability of dendritic cells. The results showed that low concentrations of PGE2 promoted cell migration, while high concentrations inhibited migration. Int J Mol Med. 2021 Jan;47(1):207-218.
Zebrafish embryos 10 µM 36 hours PGE2 treatment increased the expression of runx1/cmyb, indicating a specific role of PGE2 in AGM HSC formation. Nature. 2007 Jun 21;447(7147):1007-11.
Mouse embryonic stem cells 10 µM, 20 µM DmPGE2 increased the number of haematopoietic colonies, indicating that the role of PGE2 in regulating haematopoiesis is conserved between zebrafish and mammals. Nature. 2007 Jun 21;447(7147):1007-11.

Prostaglandin E2/前列腺素E2 动物实验

Species
Animal Model
Administration Dosage Frequency Description References
Mice EP4-KO mice Injection 10 mg/kg/d 1 month SW033291 significantly increased the number of osteoblasts and decreased the number of adipocytes in WT mice, and these effects were absent in EP4-KO mice, indicating that the PGE2/EP4 sensory nerve axis regulates MSC differentiation. J Clin Invest. 2020 Jul 1;130(7):3483-3498
Mice Tendon injury model Local injection 180 ng/week Once a week for 2 weeks PGE2 local treatment improved the biomechanical properties of repaired tendons but increased peritendinous adhesion. Adv Sci (Weinh). 2024 Oct;11(40):e2405367
C57BL/6 mice Dendritic cell migration model Footpad injection 2.5 µg/ml and 10 µg/ml Single injection, lasting 48 hours To validate the regulatory effect of PGE2 on dendritic cell migration. The results showed that high concentrations of PGE2 inhibited dendritic cell migration, while low concentrations promoted migration. Int J Mol Med. 2021 Jan;47(1):207-218.
Zebrafish Acute kidney injury model Intraperitoneal injection 2.7 μg/μL, 20 μL per fish Single injection To study the role of PGE2 in nephron regeneration, results showed that PGE2 promotes nephron regeneration through the EP4 receptor. Elife. 2023 Jan 16;12:e81438
C57Bl/6 mice Normal mice Intravenous injection 30 μg/min/kg Single administration, lasting 30 minutes PGE2 decreased cardiac contractility in mice, as evidenced by reduced +dP/dt. Circ Heart Fail. 2016 Aug;9(8):10.1161/CIRCHEARTFAILURE.116.003291 e003291.
C57BL/6 mice Myocardial infarction model Subcutaneous injection 40 µg/kg/day Daily for 2 weeks To investigate the effect of the EP3 receptor antagonist L798 106 on cardiac function post-myocardial infarction, the results showed that L798 106 significantly improved cardiac function. Circ Heart Fail. 2024 Apr;17(4):e011089
Zebrafish Sublethally irradiated zebrafish 50 μM DmPGE2 significantly enhanced kidney marrow repopulation, indicating an important role of PGE2 in kidney marrow homeostasis. Nature. 2007 Jun 21;447(7147):1007-11.

Prostaglandin E2/前列腺素E2 动物研究

Animal study 实验性偏头痛[5]
动物:恒河猴,雄性,2-4岁。
给药:0.2 mM Prostaglandin E2(与血清素(2mM)、缓激肽(2mM)和组胺(2mM)组成的炎症汤),共10 μl,导管注入硬脑膜,每三天一次,共6次。

Prostaglandin E2/前列腺素E2 临床研究

NCT号 适应症或疾病 临床期 招募状态 预计完成时间 地点
NCT00791323 - Completed - -
NCT00347503 Cataract Phase 4 Completed - United States, Pennsylvania ... 展开 >> Bucci Laser Vision Wilkes Barre, Pennsylvania, United States, 18702 收起 <<
NCT03507621 - Active, not recruiting December 15, 2018 Turkey ... 展开 >> Kahramanmaras Sutcu Imam Univercity Faculty of edicine Kahramanmaraş, Onikişubat, Turkey, 251/A 46040 收起 <<

Prostaglandin E2/前列腺素E2 参考文献

[1]Shen L, Liu Y. Prostaglandin E2 blockade enhances the pulmonary anti-Cryptococcus neoformans immune reaction via the induction of TLR-4. Int Immunopharmacol. 2015 Sep;28(1):376-81.

[2]Melin E, Lindroos E, et al. Elevated expression of prostaglandin E2 synthetic pathway in skeletal muscle of prior polio patients. J Rehabil Med. 2014 Jan;46(1):67-72.

[3]Lord AM, North TE, Zon LI. Prostaglandin E2: making more of your marrow. Cell Cycle. 2007;6(24):3054-3057.

[4]Haylor J, Towers J. Renal vasodilator activity of prostaglandin E2 in the rat anaesthetized with pentobarbitone. Br J Pharmacol. 1982;76(1):131-137.

[5]Chen N, Su W, Cui SH, Guo J, Duan JC, Li HX, He L. A novel large animal model of recurrent migraine established by repeated administration of inflammatory soup into the dura mater of the rhesus monkey. Neural Regen Res. 2019 Jan;14(1):100-106.

Prostaglandin E2/前列腺素E2 实验方案

计算器
存储液制备 1mg 5mg 10mg

1 mM

5 mM

10 mM

2.84mL

0.57mL

0.28mL

14.19mL

2.84mL

1.42mL

28.37mL

5.67mL

2.84mL

Prostaglandin E2/前列腺素E2 技术信息

CAS号363-24-6
分子式C20H32O5
分子量 352.47
SMILES Code CCCCC[C@H](O)/C=C/[C@@H]1[C@H](C(C[C@H]1O)=O)C/C=C\CCCC(O)=O
MDL No. MFCD00077861
别名 地诺前列酮 ;PGE2; Dinoprostone
运输蓝冰
InChI Key XEYBRNLFEZDVAW-ARSRFYASSA-N
Pubchem ID 5280360
存储条件

In solvent -20°C: 3-6个月 -80°C: 12个月

Pure form Sealed in dry, store in freezer, under -20°C

溶解方案

DMSO: 105 mg/mL(297.9 mM),配合低频超声助溶,注意:DMSO长时间开封后,会吸水并导致溶解能力下降,请避免使用长期开封的DMSO

请根据您的动物给药指南选择适当的溶解方案。
以下溶解方案都请先按照体外实验的方式配制澄清的储备液,再依次添加助溶剂:
——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议现用现配,当天使用; 以下溶剂前显示的百分比是指该溶剂在终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶
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