Sterile PBS or 0.9% saline for reconstitution/dilution. It is recommended to use the reconstituted/diluted product within one month.
Natalizumab/那他珠单抗 细胞实验
Cell Line
Concentration
Treated Time
Description
References
EECM-BMEC-like cells
1 μg/mL NTZ, bNTZ or mNTZ
16-24 hours
To evaluate the inhibitory effect of NTZ on the adhesion of Th1* cells to EECM-BMEC-like cells under inflammatory conditions. Results showed that NTZ alone did not significantly reduce the adhesion of Th1* cells under flow conditions, while inhibition of β2-integrins significantly reduced adhesion.
J Neuroinflammation. 2023 May 23;20(1):123
Peripheral blood mononuclear cells (PBMC)
0–120 μg/ml
40 hours
NAT exposure enhanced the production of pro-inflammatory TNF and IL-6 by B cells and CD14+ myeloid cells and upregulated the expression of CD40, CD69, and CD95 on B cells.
J Neuroinflammation. 2019 Nov 16;16(1):228
CD4+ T cells
15 μg/mL
5 days
To assess the effect of natalizumab on CD4+ T cell proliferation and activation-induced cell death. Results showed that natalizumab reduced the frequency of cell division and slightly increased activation-induced cell death.
Neurology. 2009 Jun 2;72(22):1922-30
B cells
30 μg/ml
72 hours
NAT exposure had no effect on B cell frequency, apoptosis rate, or proliferation.
J Neuroinflammation. 2019 Nov 16;16(1):228
CD4+ T cells
1, 15, 100 μg/mL
72 hours
To assess the effect of natalizumab on CD4+ T cell proliferation and IFN-γ secretion. Results showed that natalizumab at physiological concentrations reduced thymidine incorporation and IFN-γ secretion.
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