Momordin Ic (MI), a natural product isolated and purified from the fruits of Kochia scoparia (L.) Schrad., might represent a potential source of anticancer candidate, by inducing apoptosis through oxidative stress-regulated mitochondrial dysfunction involving the MAPK and PI3K-mediated iNOS and HO-1 pathways. Momordin Ic promoted the formation of autophagic vacuole and expression of Beclin 1 and LC-3 in a dose- and time-dependent manner[3]. Momordin Ic prevent cell invasion by inhibiting VCAM-1, ICAM-1, MMP-9 but inducing E-cadherin expression via p38 and JNK pathways[4]. MI inhibited cell proliferation with G0/1 phase cell cycle arrest in colon cancer cells. MI exerted an anti-tumor activity in colon cancer cells via SENP1/c-Myc signaling pathway[5]. MI, a new type of SENP1 inhibitor, reduces LPS-induced cellular inflammation by depressing SENP1 expression. Furthermore, MI-depressed Sp3 expression disturbed Sp3-nuclear factor (NF)-κB interaction and then alleviated LPS-induced cellular inflammation[6]. Momordin Ic, but not 20-hydroxyecdysone and oleanolic acid, had inhibitory effects on the production of inflammatory cytokines TNF-α and IL-6 in LPS-treated RAW264.7 macrophages[7].
Momordin Ic/地肤子皂苷Ic 细胞实验
Cell Line
Concentration
Treated Time
Description
References
MDA-MB-231 breast cancer cells
25 µM
9 days
Combined treatment with Momordin Ic and Gallic Acid significantly inhibits tumor spheroid growth
Cells. 2023 Mar 7;12(6):825
MIAPaCa-2 pancreatic cancer cells
25 µM
9 days
Combined treatment with Momordin Ic and Gallic Acid significantly inhibits tumor spheroid growth
Cells. 2023 Mar 7;12(6):825
HCT116 colon cancer cells
25 µM
9 days
Combined treatment with Momordin Ic and Gallic Acid significantly inhibits tumor spheroid growth
Cells. 2023 Mar 7;12(6):825
C-33A cervical cancer cells
25 µM
6, 12, 24, 48 hours
Momordin Ic inhibits tumor cell proliferation by promoting abnormal SUMOylation of pH3(Ser10)
Cells. 2023 Mar 7;12(6):825
CaSki cervical cancer cells
25 µM
6, 12, 24, 48 hours
Momordin Ic inhibits tumor cell proliferation by promoting abnormal SUMOylation of pH3(Ser10)
Cells. 2023 Mar 7;12(6):825
WM793
2.0 µg/mL (24 h), 1.0 µg/mL (48 h)
24 h and 48 h
Exhibits potent cytotoxicity against WM793 cells
Molecules. 2024 Aug 10;29(16):3794
A375
1.9 µg/mL (24 h), 0.9 µg/mL (48 h)
24 h and 48 h
Exhibits potent cytotoxicity against A375 cells
Molecules. 2024 Aug 10;29(16):3794
HCT-116
9.4 µg/mL (24 h), 8.3 µg/mL (48 h)
24 h and 48 h
Inhibits HCT-116 cell proliferation via suppression of SENP1/c-Myc signaling pathway
Molecules. 2024 Aug 10;29(16):3794
HT-29
4.2 µg/mL (24 h), 2.9 µg/mL (48 h)
24 h and 48 h
Inhibits HT-29 cell proliferation via suppression of SENP1/c-Myc signaling pathway
Molecules. 2024 Aug 10;29(16):3794
HepG2
12.5 µg/mL (24 h), 9.5 µg/mL (48 h)
24 h and 48 h
Induces apoptosis in HepG2 cells via MAPK- and PI3K/Akt-mediated pathways
Molecules. 2024 Aug 10;29(16):3794
Du-145
5.4 µg/mL (24 h), 2.8 µg/mL (48 h)
24 h and 48 h
Inhibits Du-145 cell proliferation via suppression of SENP1/c-Myc signaling pathway
Molecules. 2024 Aug 10;29(16):3794
PC3
5.5 µg/mL (24 h), 3.4 µg/mL (48 h)
24 h and 48 h
Inhibits PC3 cell proliferation via suppression of SENP1/c-Myc signaling pathway
Molecules. 2024 Aug 10;29(16):3794
TOV2414
24.3 µM ±13.09
5 days
Evaluate the cytotoxic effect of Momordin Ic on TOV2414 cells, IC50 was 24.3 µM ±13.09
Int J Mol Sci. 2025 Jan 8;26(2):472
MCAS
33.01 µM ±10.53
5 days
Evaluate the cytotoxic effect of Momordin Ic on MCAS cells, IC50 was 33.01 µM ±10.53
Int J Mol Sci. 2025 Jan 8;26(2):472
OCM.72
10.68 µM ±1.08
5 days
Evaluate the cytotoxic effect of Momordin Ic on OCM.72 cells, IC50 was 10.68 µM ±1.08
Int J Mol Sci. 2025 Jan 8;26(2):472
EFO27
13.19 ±1.43 µM
5 days
Evaluate the cytotoxic effect of Momordin Ic on EFO27 cells, IC50 was 13.19 ±1.43 µM
Int J Mol Sci. 2025 Jan 8;26(2):472
THP1 macrophages
2 µM
24 hours
Evaluate the inhibitory effect of Momordin Ic on THP1 macrophage infection, results showed Momordin Ic significantly inhibited HCMV infection
Pathogens. 2024 Jun 28;13(7):546
Adult retinal pigment epithelial cells (ARPE-19)
2 µM
24 hours
Evaluate the inhibitory effect of Momordin Ic on ARPE-19 cell infection, results showed Momordin Ic significantly inhibited HCMV infection
Pathogens. 2024 Jun 28;13(7):546
Human foetal foreskin fibroblasts (HFFs)
1.25 µM
24 hours
Evaluate the inhibitory effect of Momordin Ic on HCMV infection, results showed Momordin Ic significantly inhibited HCMV infection
Pathogens. 2024 Jun 28;13(7):546
Momordin Ic/地肤子皂苷Ic 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Mice
Pressure overload-induced cardiac hypertrophy model
Intraperitoneal injection
10 mg/kg
Once daily for 20 consecutive days
Momordin Ic accelerated pressure overload-induced cardiac remodeling
J Am Heart Assoc. 2022 Nov 15;11(22):e027004
NOD/SCID mice
AML xenograft model
Intraperitoneal injection
10 mg/kg
Ara-C for 5 consecutive days, Momordin Ic every two days for a week
To evaluate the effect of Momordin Ic combined with Ara-C on AML xenograft model, results showed that combination therapy significantly reduced AML cell survival and improved mouse survival.
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