Jujuboside A, a natural product isolated and purified from the seeds of Zizyphus jujuba with neurophysiological inhibitory effects, performs its specific sedative and hypnotic effect through not only adjusting GABA receptors subunit mRNAs expression, but also down-regulating the secretion of relevant inflammation cytokines on the intestinal mucosal system to affect the intercellular cytokine network between nerve cells in the brain and may serve as a potential therapeutic agent for the treatment of Alzheimer׳s disease.
Jujuboside A/酸枣仁皂苷 A 细胞实验
Cell Line
Concentration
Treated Time
Description
References
B16F10 melanoma cells
20 µM
96 hours
To evaluate the effect of JUA on the viability of B16F10 cells, results showed no significant effect on cell viability.
Int J Mol Sci. 2021 Jul 19;22(14):7701
SK-N-SH cells
4, 8, 16 μM
24 hours
Jujuboside A significantly reversed the cell viability suppressed by 25 μM of 6-OHDA at 4, 8, and 16 μM to 60.50±2.66%, 73.50±5.13%, and 79.83±3.06%.
Molecules. 2022 Jun 26;27(13):4106
SH-SY5Y cells
4, 8, 16 μM
24 hours
Jujuboside A significantly reversed the cell viability suppressed by 25 μM of 6-OHDA at 4, 8, and 16 μM to 59.83±4.54%, 72.67±4.84%, and 86.50±3.83%.
Molecules. 2022 Jun 26;27(13):4106
Primary microglia
1 µM, 5 µM, 25 µM
12 hours
JuA pretreatment reversed Aβ42-induced HSP90β and PPARγ decrease in a dose-dependent manner.
Theranostics. 2018 Jul 30;8(15):4262-4278
BV2 cells
1 µM, 5 µM, 25 µM
12 hours
JuA pretreatment reversed Aβ42-induced HSP90β and PPARγ decrease in a dose-dependent manner.
Theranostics. 2018 Jul 30;8(15):4262-4278
Jujuboside A/酸枣仁皂苷 A 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
APP/PS1 transgenic mice
Alzheimer's disease model
Intrathecal injection
0.5 mg/kg, 1.5 mg/kg, 5 mg/kg
Once daily for 7 days
JuA significantly ameliorated cognitive deficiency in APP/PS1 transgenic mice, meanwhile, JuA significantly reduced the soluble Aβ42 levels and plaque numbers in the brain.
To evaluate the effects of jujuboside A, jujuboside B, and spinosin on cognitive function in mice. The results showed that the mixture had a much weaker effect than 0.5 mg ZSS hot water extract.
Elife. 2025 Apr 23;13:RP100737
Zebrafish larvae
Α-MSH-induced pigmentation model
20 µM
72 hours
To evaluate the effect of JUA on α-MSH-induced pigmentation in zebrafish larvae, results showed no significant anti-melanogenic effect.
Int J Mol Sci. 2021 Jul 19;22(14):7701
Sprague-Dawley rats
Chronic unpredictable mild stress (CUMS)-induced depression model
Oral
12.5 mg/kg, 25 mg/kg, 50 mg/kg
Once daily for 4 weeks
To evaluate the effect of JuA on depressive-like behavior and cognitive function in CUMS rats, results showed that JuA significantly ameliorated depressive-like behavior and cognitive dysfunction, increased monoamine neurotransmitter levels in serum and hippocampal tissue, reduced the number of BrdU/DCX-positive immature neurons, and attenuated calcium ion (Ca2+) concentration and CaMKII levels in immature neurons.
Drug Des Devel Ther. 2024 Oct 12;18:4565-4584
ICR mice
Pentobarbital-induced sleep model
Intraperitoneal injection
5 mg/kg and 10 mg/kg
1 h and 25 h before
JuA significantly prolonged pentobarbital-induced sleep duration without affecting sleep latency.
Nutrients. 2024 Dec 11;16(24):4266
Kunming mice
Healthy Kunming mice
Intragastric administration
10/20/30 mg/kg/d
Once daily for 21 days
Assess sleep state and neurotransmitter levels, results showed JuA significantly improved sleep state
Aging (Albany NY). 2023 Sep 5;15(18):9426-9437
APP/PS1 transgenic mice
Alzheimer's disease mouse model
Intracerebroventricular injection
0.02 mg/kg
Five consecutive days, once daily
JuA prevents sleep loss-induced disturbance of hippocampal neuronal excitability and memory impairment via GABAergic mechanism.
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