Isoalantolactone, a sesquiterpene lactone compound that can be purified from the roots of Inula helenium L, with antifungal, anti-bacterial, anti-helminthic and anti-proliferative properties. In vitro, Isoalantolactone enhanced the radiosensitivity of UMSCC-10A cells; the sensitivity enhanced ratios (SERs) were 1.44 and 1.63, respectively, for 2.5 and 5 μM[5]. Treating SK-MES-1 cells with 0, 20, and 40 M of Isoalantolactone for 24 h change its morphology: rounded and shrunken cells, and decreased number of cells, the IC50 values were about 40 μM after 24 h treatment[4].
Isoalantolactone/异土木香内酯 细胞实验
Cell Line
Concentration
Treated Time
Description
References
DU145 cells
2.5, 5, 10, 20, 30, 40, 50 and 60 μM
24 h
IATL dose-dependently inhibited the growth of DU145 cells and induced apoptosis.
J Exp Clin Cancer Res. 2018 Dec 12;37(1):309.
PC-3 cells
2.5, 5, 10, 20, 30, 40, 50 and 60 μM
24 h
IATL dose-dependently inhibited the growth of PC-3 cells and induced apoptosis.
J Exp Clin Cancer Res. 2018 Dec 12;37(1):309.
COS-7 cells
20 and 40 µM
24 h
To evaluate the toxicity of Isoalantolactone on normal COS-7 cells. Results showed that Isoalantolactone had significantly lower toxicity on COS-7 cells compared to PANC-1 cells.
Int J Biol Sci. 2012;8(4):533-47.
HPAC cells
20 and 40 µM
24 h
To evaluate the effect of Isoalantolactone on growth inhibition in HPAC cells. Results showed that Isoalantolactone inhibited HPAC cell growth in a dose-dependent manner.
Int J Biol Sci. 2012;8(4):533-47.
BxPC3 cells
20 and 40 µM
24 h
To evaluate the effect of Isoalantolactone on growth inhibition in BxPC3 cells. Results showed that Isoalantolactone inhibited BxPC3 cell growth in a dose-dependent manner.
Int J Biol Sci. 2012;8(4):533-47.
PANC-1 cells
20 and 40 µM
24 h
To evaluate the effect of Isoalantolactone on growth inhibition and apoptosis in PANC-1 cells. Results showed that Isoalantolactone inhibited PANC-1 cell growth and induced apoptosis in a dose-dependent manner.
Int J Biol Sci. 2012;8(4):533-47.
HEK293 cells
10 μM
6 h
Inhibited K63-linked polyubiquitination of TRAF6
Acta Pharmacol Sin. 2019 Jan;40(1):64-74.
mouse bone marrow-derived macrophages (BMDMs)
2.5, 5, 10, 20 μM
12 h
Inhibited LPS-induced NO production and iNOS expression
Acta Pharmacol Sin. 2019 Jan;40(1):64-74.
BV2 cells
1 μM and 2 μM
12 h
IAL inhibited LPS-induced release of pro-inflammatory mediators in BV2 cells by activating the AKT/Nrf2/HO-1 pathway and inhibiting the NF-κB pathway
Cells. 2022 Sep 19;11(18):2927.
SN4741 cells
2 μM
24 h
IAL regulated apoptosis-related proteins by activating the AKT/Nrf2 pathway, thereby suppressing the apoptosis of SN4741 cells induced by MPP+
Cells. 2022 Sep 19;11(18):2927.
PC-3 cells
15 μM
24 h
IATL and cisplatin combination significantly suppressed PC-3 cell growth and induced apoptosis.
Front Cell Dev Biol. 2021 Apr 20;9:632779.
DU145 cells
15 μM
24 h
IATL and cisplatin combination significantly suppressed DU145 cell growth and induced apoptosis.
Front Cell Dev Biol. 2021 Apr 20;9:632779.
HEK293T cells
100 μM
30 min
To detect the direct interaction between Isoalantolactone and survivin protein by CETSA, the results showed that Isoalantolactone might induce thermal stability changes of survivin protein by direct binding.
Cell Cycle. 2023 Jun;22(12):1407-1420.
K562R cells
2.5, 5, 10, 20, 40, 80 μM
0, 12, 24, 36, 48 h
To evaluate the anti-proliferative effect of Isoalantolactone on K562R cells, the results showed that Isoalantolactone inhibited the proliferation of K562R cells in a dose- and time-dependent manner, with an IC50 value of 19.32 μM at 48 h.
Cell Cycle. 2023 Jun;22(12):1407-1420.
K562 cells
2.5, 5, 10, 20, 40, 80 μM
0, 12, 24, 36, 48 h
To evaluate the anti-proliferative effect of Isoalantolactone on K562 cells, the results showed that Isoalantolactone inhibited the proliferation of K562 cells in a dose- and time-dependent manner, with an IC50 value of 5.04 μM at 48 h.
Cell Cycle. 2023 Jun;22(12):1407-1420.
KBM5T315I cells
2.5, 5, 10, 20, 40, 80 μM
0, 12, 24, 36, 48 h
To evaluate the anti-proliferative effect of Isoalantolactone on KBM5T315I cells, the results showed that Isoalantolactone inhibited the proliferation of KBM5T315I cells in a dose- and time-dependent manner, with an IC50 value of 15.13 μM at 48 h.
Cell Cycle. 2023 Jun;22(12):1407-1420.
KBM5 cells
2.5, 5, 10, 20, 40, 80 μM
0, 12, 24, 36, 48 h
To evaluate the anti-proliferative effect of Isoalantolactone on KBM5 cells, the results showed that Isoalantolactone inhibited the proliferation of KBM5 cells in a dose- and time-dependent manner, with an IC50 value of 8.58 μM at 48 h.
Cell Cycle. 2023 Jun;22(12):1407-1420.
OVCAR-3 cells
10-50 μM
24 h
IL inhibited OVCAR-3 cell proliferation and induced G2/M phase cell cycle arrest
Acta Biochim Biophys Sin (Shanghai). 2023 Feb 25;55(1):62-71.
SKOV-3 cells
10-50 μM
24 h
IL inhibited SKOV-3 cell proliferation and induced G2/M phase cell cycle arrest
Acta Biochim Biophys Sin (Shanghai). 2023 Feb 25;55(1):62-71.
Isoalantolactone/异土木香内酯 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Nude mice (nu/nu)
DU145 xenograft model
Intraperitoneal injection
10 mg/kg
Once every other day for 49 days
IATL significantly inhibited the growth of DU145 xenograft tumors without exhibiting toxicity.
J Exp Clin Cancer Res. 2018 Dec 12;37(1):309.
CD1 mice
Acute and chronic toxicity model
Intraperitoneal injection
100 mg/kg
Once daily for 7 or 30 days
To evaluate the acute and chronic toxicity of Isoalantolactone in CD1 mice. Results showed that Isoalantolactone did not induce any acute or chronic toxicity at a dose of 100 mg/kg body weight.
Int J Biol Sci. 2012;8(4):533-47.
C57/BL6 mice
LPS-induced acute lung injury (ALI) model
Intraperitoneal injection
20 mg/kg
Once
Alleviated pulmonary pathological changes, neutrophil infiltration, and pro-inflammatory cytokine expression
Acta Pharmacol Sin. 2019 Jan;40(1):64-74.
C57/BL6 mice
MPTP-induced Parkinson's disease model
Gavage
10 mg/kg
17 days
IAL ameliorated MPTP-induced PD-related pathological impairment and decreased motor activity in mice
Cells. 2022 Sep 19;11(18):2927.
Nude mice
DU145 xenograft model
Intraperitoneal injection
5 mg/kg
Once every 3 days, continuous treatment
IATL and cisplatin combination significantly inhibited tumor growth and weight.
Front Cell Dev Biol. 2021 Apr 20;9:632779.
C57BL/6 mice
Chronic social defeat stress (CSDS) model
Oral gavage
10 mg/kg
Once daily for 5 days
To evaluate the effects of LAT on CSDS-induced depression-like behaviors in mice. Results showed that LAT significantly improved depression-like behaviors, restored prefrontal cortex protein expression, reduced inflammatory cytokines IL-6 and TNF-α levels, and modulated gut microbiota.
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