IMP-1088 is a potent dual inhibitor of HsNMT1/2 with IC50 values of less than 1 nM, and the Kd value of IMP-1088 against HsNMT1 is less than 210 pM. IMP-1088 effectively blocks rhinovirus replication by blocking the N-myristoylation of rhinovirus-encoded protein (VP0). IMP-1088 protects host cells from cytotoxicity caused by viral infection. At concentrations less than 1000 nM, IMP-1088 prevented virus-induced cytopathic effects in a dose-dependent manner, with IC50 values of 17 nM, respectively, and at a concentration of 125 nM, IMP-1088 completely inhibited newly infected viruses, with an IC50 value of 5.8 nM. IMP-1088 blocks infectious virus production and inhibits single-cycle replication 7 hours after infection of primary human bronchial epithelial cells (hBEC) with rhinovirus RV-A1 (MOI 5). IMP-1088 significantly inhibits the production of infectious virus even within 3 hours of infection[1].
IMP-1088 细胞实验
Cell Line
Concentration
Treated Time
Description
References
HeLa cells
125 nM
6 hours
Complete suppression of infectious virus production
Nat Chem. 2018 Jun;10(6):599-606
HeLa cells
50 nM
6 hours
Inhibition of RV-A16 VP0 myristoylation post-infection
Nat Chem. 2018 Jun;10(6):599-606
human fibroblasts
100 nM
To study the inhibitory effect of IMP-1088 on N-myristoylation
Brain. 2024 Apr 4;147(4):1436-1456
HeLa
1 nM, 10 nM, 100 nM
24 hours
To evaluate the inhibitory effect of IMP-1088 on NMT activity, results showed that IMP-1088 significantly inhibited NMT activity.
Mol Cell Proteomics. 2019 Jan;18(1):115-126
Panc-1
1 nM, 10 nM, 100 nM
24 hours
To evaluate the inhibitory effect of IMP-1088 on NMT activity, results showed that IMP-1088 significantly inhibited NMT activity.
Mol Cell Proteomics. 2019 Jan;18(1):115-126
MDA-MB-231
1 nM, 10 nM, 100 nM
24 hours
To evaluate the inhibitory effect of IMP-1088 on NMT activity, results showed that IMP-1088 significantly inhibited NMT activity.
Mol Cell Proteomics. 2019 Jan;18(1):115-126
HeLa cells
500 nM
6 hours
Blockade of viral assembly, preventing infectious virion formation
Nat Chem. 2018 Jun;10(6):599-606
ST cells
50 nM and 100 nM
24 hours
To evaluate the effect of IMP-1088 on SVA replication, results showed that IMP-1088 significantly inhibited SVA replication.
Pathogens. 2024 Jul 21;13(7):601
BHK-21 cells
100 nM
24 hours
To evaluate the effect of IMP-1088 on SVA replication, results showed that IMP-1088 significantly inhibited SVA replication.
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