Glabridin, a compound in the root extract of Glycyrrhiza glabra, as an effective tyrosinase inhibitor. Glabridin applied on skin can inhibit melanin synthesis[3]. Glabridin significantly inhibited migration/invasion capacities of HCC cells, Huh7 and Sk-Hep-1[6]. Glabridin-induced cell apoptosis in HL-60 and MV4-11 cells[7]. Glabridin also upregulated the expression of miR-148a in a dose-dependent manner and attenuated angiogenesis in two human breast cancer cell lines (MDA-MB-231 and Hs-578T)[5]. Glabridin((10 or 50 mg/kg/day) ) could ameliorate dextran sulphate sodium (DSS)-induced colitis[8].
Glabridin/光甘草定 细胞实验
Cell Line
Concentration
Treated Time
Description
References
Peripheral blood mononuclear cells (PBMCs)
20 μM
1 hour
Glabridin effectively inhibited the activation of IRF3 in PBMCs and reduced the expression of IFN-β, IL-6, and TNF-α.
Mol Med. 2023 Dec 8;29(1):167
Bone-marrow-derived macrophages (BMDMs)
20 μM
1 hour
Glabridin significantly inhibited the activation of STING and IRF3 in BMDMs and reduced the expression of IFN-β, IL-6, and TNF-α.
Mol Med. 2023 Dec 8;29(1):167
RAW264.7 cells
10 μM and 20 μM
24 hours
To evaluate the anti-inflammatory effect of GLA, results showed that GLA significantly inhibited NO secretion by macrophages.
Adv Sci (Weinh). 2025 May;12(18):e2407802
Peripheral blood mononuclear cells (PBMCs)
20 μM
1 hour
To verify the broad-spectrum inhibitory effect of Glabridin on the cGAS-STING signaling pathway. Results showed that Glabridin effectively inhibited cGAMP and diABZI-induced IRF3 activation and reduced the gene expression of IFN-β, IL-6, and TNF-α.
Mol Med. 2023 Dec 8;29(1):167
Bone-marrow-derived macrophages (BMDMs)
20 μM
1 hour
To evaluate the effect of Glabridin on the cGAS-STING signaling pathway. Results showed that Glabridin significantly inhibited HT-DNA-induced activation of STING and IRF3, and reduced the gene expression of IFN-β, IL-6, and TNF-α.
Mol Med. 2023 Dec 8;29(1):167
T. gondii RH strain tachyzoites
5 µg/ml
24 and 48 hours
To evaluate the inhibitory effects of glabridin on T. gondii tachyzoites, results showed significant inhibition of proliferation and invasion, along with structural damage.
Parasit Vectors. 2024 Dec 18;17(1):522
Vero cells
5 µg/ml
24 hours
To evaluate the cytotoxicity of glabridin on Vero cells, results showed 99.05% cell viability at 5 µg/ml with no significant toxicity.
Parasit Vectors. 2024 Dec 18;17(1):522
Human platelets
5–30 µM
3 minutes
Inhibited collagen-induced platelet aggregation
Chin Med. 2023 Jun 10;18(1):71
Human cutaneous T-cell lymphoma cell line H9
0, 2.5, 5, 10, 20, 40, 80 μM
24 hours
To evaluate the effect of Glabridin on CTCL cell viability, results showed that Glabridin significantly inhibits cell viability and induces programmed cell death (apoptosis, autophagy, and necrosis).
Cell Prolif. 2024 Sep;57(9):e13701
Human cutaneous T-cell lymphoma cell line HH
0, 2.5, 5, 10, 20, 40, 80 μM
24 hours
To evaluate the effect of Glabridin on CTCL cell viability, results showed that Glabridin significantly inhibits cell viability and induces programmed cell death (apoptosis, autophagy, and necrosis).
Cell Prolif. 2024 Sep;57(9):e13701
Human platelets
20 and 30 µM
3 minutes
Inhibited phosphorylation of Lyn, Fyn, Syk, and integrin β3
Chin Med. 2023 Jun 10;18(1):71
Glabridin/光甘草定 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
C57BL/6J mice
Trex1-deficient mice
Intraperitoneal injection
40 mg/kg
Daily for 14 days
Glabridin significantly alleviated the inflammatory response in Trex1-deficient mice and reduced the expression of IFN-β, CXCL10, ISG15, IL-6, and TNF-α in various tissues.
Mol Med. 2023 Dec 8;29(1):167
C57BL/6J mice
DMXAA-induced cGAS-STING signaling pathway activation model
Intraperitoneal injection
20 or 40 mg/kg
Single injection, lasting 4 hours
To study the inhibitory effect of Glabridin on the cGAS-STING signaling pathway in vivo. Results showed that Glabridin significantly reduced the levels of IFN-β, IL-6, and TNF-α in serum and peritoneal lavage fluid induced by DMXAA.
Mol Med. 2023 Dec 8;29(1):167
Sprague-Dawley rats
High-fat diet combined with streptozotocin-induced diabetic nephropathy model
Intraperitoneal injection
50 mg/kg
Once daily for 28 consecutive days
To investigate the protective effect of Glabridin on diabetic nephropathy in rats, results showed that Glabridin could improve renal function, alleviate renal pathological changes, inhibit oxidative stress and ferroptosis, and exert its effects by regulating the VEGF/Akt/ERK pathway.
Mol Med. 2022 May 20;28(1):58
BALB/c mice
T. gondii RH strain infection model
Oral gavage
50 mg/kg and 100 mg/kg
7 consecutive days
To evaluate the effect of glabridin on the survival rate of T. gondii-infected mice, results showed that 100 mg/kg dose significantly prolonged survival time, with 20% of mice surviving until day 25 post-infection.
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