Ganoderic acid A (GAA), a representative active triterpenoid from Ganoderma lucidum, has been reported to exhibit antinociceptive, antioxidative, cytotoxic, hepatoprotective and anticancer activities[3]. GA-A (6.25-100 μM) significantly inhibited cyst growth in MDCK cyst model and embryonic kidney cyst model in vitro, and the inhibitory effect was reversible. In kidney-specific Pkd1 knockout (kPKD) mice displaying severe cystic kidney disease, administration of GA-A (50 mg· kg-1 ·d-1, sc) significantly attenuated renal cyst development[4]. Pretreatment with GA A (10 μM) significantly attenuated SNP(sodium nitroprusside)-induced cytotoxicity and NO (nitric oxide) increase in SH-SY5Y cells, but not in PC12 cells. Furthermore, pretreatment with GA A caused significantly higher adrenaline content in SH-SY5Y cells than in PC12 cells[5]. GAA attenuates acute lung injury in mouse model via the inhibition of Rho/ROCK/NF-κB pathway[6].
Ganoderic acid A/灵芝酸 A 细胞实验
Cell Line
Concentration
Treated Time
Description
References
HASMCs
10 µM
7 days
To evaluate the effect of Ganoderic acid A on vascular smooth muscle cell calcification. Results showed that Ganoderic acid A significantly reduced calcium deposition.
Theranostics. 2023 Feb 21;13(4):1325-1341.
RAW264.7 cells
10 µM
24 hours
To evaluate the effect of Ganoderic acid A on foam cell formation. Results showed that Ganoderic acid A significantly reduced the number of foam cells.
Theranostics. 2023 Feb 21;13(4):1325-1341.
Embryonic kidney cells
100μM
6 days
Assess the inhibitory effect of GA-A on embryonic kidney cysts, showing significant reduction in cyst number and size.
Acta Pharmacol Sin. 2020 Jun;41(6):782-790.
MDCK cells
6.25-100μM
24 hours
Evaluate the inhibitory effect of GA-A on cyst growth in MDCK cells, showing significant inhibition without cytotoxicity.
To evaluate the anti-proliferative activity of GA-A, results showed that GA-A significantly reduced cell viability in primary B-cell lymphomas with less effect on healthy B-cells.
J Cell Biochem. 2015 Jan;116(1):102-14.
Non-Hodgkin’s lymphoma cells (DB, Toledo)
5-40μM
24 hours
To evaluate the anti-proliferative activity of GA-A, results showed that GA-A significantly reduced cell viability with an IC50 of approximately 15μM.
J Cell Biochem. 2015 Jan;116(1):102-14.
Human pre-B acute lymphocytic leukemia cells (NALM-6)
5-40μM
24 hours
To evaluate the anti-proliferative activity of GA-A, results showed that GA-A significantly reduced cell viability with an IC50 of approximately 15-22μM.
J Cell Biochem. 2015 Jan;116(1):102-14.
BV2 microglial cells
20 μM
6 hours
Increases phosphorylation of Axl and Pak1, activating autophagy
Int J Mol Sci. 2021 May 24;22(11):5559.
BV2 microglial cells
50 μg/ml
24 hours
To evaluate the inhibitory effect of GAA on LPS-induced proliferation and activation of BV2 microglial cells. Results showed that GAA significantly suppressed LPS-induced BV2 microglial cells proliferation and activation, and promoted their conversion from M1 to M2 status.
Neurochem Res. 2021 Jul;46(7):1725-1736.
SNU719 cells
0.49 mM
48 hours
To evaluate the cytotoxic and apoptosis-inducing effects of GAA and QCT co-treatment on SNU719 cells. Results showed that the co-treatment significantly enhanced QCT-mediated cytotoxicity and apoptosis.
Molecules. 2019 Oct 24;24(21):3834.
Ganoderic acid A/灵芝酸 A 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Mice
KPKD mouse model
Subcutaneous injection
50 mg/kg/d
Once daily for 4 days
Evaluate the inhibitory effect of GA-A on renal cysts in kPKD mice, showing significant reduction in cyst area and kidney index.
Acta Pharmacol Sin. 2020 Jun;41(6):782-790.
C57BL/6 mice
EL4 syngeneic mouse lymphoma model
Intraperitoneal injection
60, 50 and 50 mg/kg body weight
Injected once on days 10, 14, and 18
To evaluate the effect of GA-A on lymphoma growth and metastasis, results showed that GA-A significantly prolonged survival, reduced liver metastasis, and decreased the number of MDSCs.
Ganoderic acid A alleviates OVA-induced asthma inflammation in mice by inhibiting the TLR/NF-κB signaling pathway.
Inflammation. 2021 Oct;44(5):1908-1915
BALB/c mice
LPS-induced acute lung injury model
Intraperitoneal injection
10 mg/kg and 20 mg/kg
Single dose, lasted for 6 hours
To investigate the protective effects of ganoderic acid A (GAA) on LPS-induced acute lung injury and its mechanism. The results showed that GAA significantly reduced lung wet-to-dry weight ratio, lung myeloperoxidase activity, attenuated pathological damages, increased superoxide dismutase activity, and decreased malondialdehyde content and proinflammatory cytokine levels in bronchoalveolar lavage fluid. Mechanistically, GAA inhibited LPS-induced inflammation by suppressing the Rho/ROCK/NF-κB pathway.
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