Fraxetin, a natural product isolated and purified from the bark of Fraxinus brngeana DC., has protective against rotenone-induced cytotoxicity, apoptotic death and antioxidant activities.
Fraxetin/秦皮素 细胞实验
Cell Line
Concentration
Treated Time
Description
References
Yeast microsomes
100 μM fraxetin
1 hour
Validate CYP82C4's ability to convert fraxetin to sideretin
Nat Chem Biol. 2018 May;14(5):442-450
Patu8988 cells
50 or 100 μM
24 hours
To evaluate the effect of Fraxetin on the proliferation of Patu8988 cells. Results showed that Fraxetin inhibited the proliferation of Patu8988 cells in a concentration-dependent manner.
Aging (Albany NY). 2021 Jul 28;13(14):18545-18563
PANC-1 cells
50 or 100 μM
24 hours
To evaluate the effect of Fraxetin on the proliferation of PANC-1 cells. Results showed that Fraxetin inhibited the proliferation of PANC-1 cells in a concentration-dependent manner.
Aging (Albany NY). 2021 Jul 28;13(14):18545-18563
prostate cancer cell DU145
10, 20, 40 μM
48 hours
To evaluate the effect of Fraxetin on cell migration and invasion, results showed that Fraxetin significantly inhibited DU145 cell migration and invasion
Bioengineered. 2022 Apr;13(4):9345-9356
prostate cancer cell DU145
10, 20, 40 μM
48 hours
To evaluate the effect of Fraxetin on cell apoptosis, results showed that Fraxetin significantly increased the ratio of apoptotic DU145 cells
Bioengineered. 2022 Apr;13(4):9345-9356
prostate cancer cell DU145
10, 20, 40 μM
24, 48, 72 hours
To evaluate the effect of Fraxetin on cell proliferation, results showed that Fraxetin significantly inhibited DU145 cell proliferation
Bioengineered. 2022 Apr;13(4):9345-9356
prostate cancer cell DU145
10, 20, 40, 80 μM
48 hours
To evaluate the effect of Fraxetin on cell viability, results showed that Fraxetin significantly reduced DU145 cell viability
Bioengineered. 2022 Apr;13(4):9345-9356
prostatic epithelial cell RWPE-1
10, 20, 40, 80 μM
48 hours
To evaluate the effect of Fraxetin on cell viability, results showed that Fraxetin had no obvious effect on RWPE-1 cell viability
Bioengineered. 2022 Apr;13(4):9345-9356
HL-60 cells
0, 20, 40, 80, 160 µM
24, 48, 72 hours
To evaluate the inhibitory effect of Fraxetin on the proliferation of HL-60 cells. The results showed that the viability of HL-60 cells was inhibited at different time points as the concentration of fraxetin increased.
BMC Complement Med Ther. 2024 Jun 10;24(1):226
THP-1 cells
0, 20, 40, 80, 160 µM
24, 48, 72 hours
To evaluate the inhibitory effect of Fraxetin on the proliferation of THP-1 cells. The results showed that the viability of THP-1 cells was inhibited at different time points as the concentration of fraxetin increased.
BMC Complement Med Ther. 2024 Jun 10;24(1):226
3T3-L1 cells
0, 5, 10, 20, 50, 100 µM
2 days
To evaluate the effect of Fraxetin on adipogenesis and lipid accumulation. Results showed that Fraxetin significantly inhibited lipid droplet accumulation in 3T3-L1 cells without cytotoxicity.
Antioxidants (Basel). 2022 Sep 24;11(10):1893
HL-60 cells
40 µM, 80 µM, 120 µM
48 hours
To evaluate the apoptotic effect of Fraxetin on HL-60 cells. The results showed that the apoptosis rate of HL-60 cells gradually increased with increasing concentrations of fraxetin.
BMC Complement Med Ther. 2024 Jun 10;24(1):226
THP-1 cells
50 µM, 100 µM, 150 µM
48 hours
To evaluate the apoptotic effect of Fraxetin on THP-1 cells. The results showed that the apoptosis rate of THP-1 cells gradually increased with increasing concentrations of fraxetin.
BMC Complement Med Ther. 2024 Jun 10;24(1):226
Fraxetin/秦皮素 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Nude mice (BALB/c)
Pancreatic ductal adenocarcinoma (PDA) xenograft model
Intragastric administration
25 mg/kg·d
Every 3 days for one month
To evaluate the effect of Fraxetin on tumor growth and metastasis in PDA xenograft models. Results showed that Fraxetin significantly inhibited tumor growth and metastasis.
Aging (Albany NY). 2021 Jul 28;13(14):18545-18563
Arabidopsis thaliana
Fe-deficient Arabidopsis
Media supplementation
120 μM fraxetin
14 days
Partially rescued the s8h mutants, indicating that fraxetin is a bioactive compound participating in the acquisition of Fe from immobile sources.
Plant Physiol. 2018 May;177(1):194-207
Arabidopsis thaliana
F6’h1, s8h, cyp82C4 mutants
Agar medium
150 μM
6-hour treatment, lasting 7 days
Validate the rescue effect of fraxetin and sideretin on iron deficiency phenotypes
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