Columbianadin, a natural product isolated and purified from the herbs of Angelicae pubescens with analgesic, anti-inflammatory, calcium-channel blocking, and platelet aggregation inhibiting functions, can effectively suppress the growth of colon cancer cells, low concentration (up to 25 μM) of CBN induces apoptosis, and high concentration (50 μM) of CBN induces necroptosis, demonstrates that CBN has the potential to be a candidate in the development of anti-cancer agent derived from natural products.
Columbianadin/二氢欧山芹醇当归酸酯 细胞实验
Cell Line
Concentration
Treated Time
Description
References
HL-1 cardiomyocytes
10 µM
Inhibited peak INa and increased inactivation rate of the current
Int J Mol Sci. 2021 Jan 9;22(2):621.
RAW 264.7 cells
10–180 μM
24 h
To evaluate the effect of CBN on cell viability and inflammatory response. Results showed that CBN had no significant effect on cell viability up to 60 μM and significantly inhibited LPS-induced inflammatory responses.
Antioxidants (Basel). 2021 Apr 2;10(4):553
GH3 cells
14.7 µM (peak INa), 2.8 µM (late INa)
1 minute
Inhibited peak and late components of voltage-gated Na+ current
Int J Mol Sci. 2021 Jan 9;22(2):621.
HCT116 human colorectal cancer cells
12.5, 25, 50 μM
48 h
To investigate the inhibitory effect of CBN on HCT116 cell proliferation and its mechanism. Results showed that CBN induced apoptosis at low concentration (25 μM) and necroptosis at high concentration (50 μM).
Biomol Ther (Seoul). 2016 May 1;24(3):320-7
human platelets
40–100 μM
3 min
inhibited collagen-induced platelet aggregation
J Biomed Sci. 2020 May 6;27(1):60.
Columbianadin/二氢欧山芹醇当归酸酯 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
C57BL/6 mice
LPS-induced acute liver injury model
Intraperitoneal injection
10 and 20 mg/kg
Single dose, lasting 6 hours
To evaluate the protective effect of CBN on LPS-induced liver injury. Results showed that CBN significantly reduced serum ALT and AST activities and alleviated histopathological changes in liver tissues.
Antioxidants (Basel). 2021 Apr 2;10(4):553
ICR mice
D-Galactose-induced liver injury model
Intraperitoneal injection and oral
200, 400, 800 mg/kg body weight
Once daily for 8 weeks
To study the protective effect of CBN against D-galactose-induced liver injury and its mechanism. Results showed that CBN significantly ameliorated liver injury, reduced inflammatory response and oxidative stress
Pharmaceuticals (Basel). 2024 Mar 15;17(3):378
Mice
Mesenteric microvascular thrombosis model
Intravenous injection
5 and 10 mg/kg
Single dose
Prolonged thrombotic occlusion time and reduced ADP-induced acute pulmonary embolism mortality
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