There will be a HazMat fee per item when shipping a dangerous goods. The HazMat fee will be charged to your UPS/DHL/FedEx collect account or added to the invoice unless the package is shipped via Ground service. Ship by air in Excepted Quantity (each bottle), which is up to 1g/1mL for class 6.1 packing group I or II, and up to 25g/25ml for all other HazMat items.
Type
HazMat fee for 500 gram (Estimated)
Excepted Quantity
USD 0.00
Limited Quantity
USD 15-60
Inaccessible (Haz class 6.1), Domestic
USD 80+
Inaccessible (Haz class 6.1), International
USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic
USD 100+
Accessible (Haz class 3, 4, 5 or 8), International
Carmustine is an antitumor chemotherapeutic agent, which works by akylating DNA and RNA. NAT (N-acetyltransferase) activity in glial tumor cell cytosols and intact tumor cells were decreased by carmustine in a dose-dependent manner. Carmustine (8, 80, and 800 µM) decreases N-acetyltransferase (NAT) activities for 2-aminofluorene (AF) and p-aminobenzoic acid (PABA) in rat glial tumor cytosol and intact cells. In rat glial tumor cells, the DNA-AF adduct increases, and carmustine decreases the formation of DNA-AF adduct[3]. Preincubation of cells with the glutathione reductase inhibitor, carmustine, led to elevated basal [Ca2+]i, yet the cells remained responsive to bradykinin[4]. Carmustine (≥25 µM) stimulates eryptosis at least partially by increasing cytosolic Ca²⁺ activity[5]. Carmustine (BCNU; 25 mg/kg, i.p.) causes higher levels of the rhe ratio of liver weight to body weight and plasma conjugated bilirubin, and lower biliary flow, oxidised glutation levels (GSSG) and reduced glutation (GSH)/GSSG values compared with control rats[6].
Carmustine/卡莫司汀 细胞实验
Cell Line
Concentration
Treated Time
Description
References
Murine B16 melanoma cells
10-30 µM
1 hour
To evaluate the cytotoxicity of BCNU alone and in combination with TNF on B16 cells. Results showed BCNU reduced cell survival at 10-30 μM, with 30 μM reducing survival to 0.1 of control. Addition of TNF (2000 U/ml) further reduced survival at all BCNU doses, with 30 μM BCNU + TNF achieving one-tenth the survival of BCNU alone.
Br J Cancer. 1990 Nov;62(5):776-80.
Neuro-2A (N2a) cells
0-240 µM
24 hours
BCNU was non-toxic up to 20 μM but showed significant cytotoxicity at 80 μM (69.5%) and 240 μM (49%).
BMC Med. 2013 Mar 26;11:81.
Carmustine/卡莫司汀 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Mice
APdE9 mice (Alzheimer’s disease model)
Intraperitoneal injection
0.5 mg/kg
Daily for 60 days
Chronic BCNU administration reduced Aβ40 levels by 75% and amyloid plaque burden by 81%. Conversely, sAPPα levels were increased by 45%. BCNU also reduced microglial activation (30% reduction in hippocampus and 44% reduction in motor cortex).
BMC Med. 2013 Mar 26;11:81.
Mice
Cerebellar cortical dysplasia model
Intraperitoneal injection
20 mg/kg
Single injection on embryonic day 13
To assess the effect of carmustine on cerebellar cortical development in mice. Results showed that carmustine treatment caused disorganization of cerebellar cortical layers, altered morphology of Bergmann glia and astrocytes, and impaired motor coordination. Additionally, spontaneous calcium signaling activity in Bergmann glia increased but with shorter duration.
Cells. 2021 Jun 23;10(7):1581
C57BL/6 mice
B16F10 xenograft model
Intraperitoneal injection
35 mg/kg
Every 2 days for 14 days
To evaluate the antitumor effects of carmustine on B16F10 xenografts. Results showed that carmustine significantly inhibited tumor growth and enhanced the antitumor effects of BCNU and CCNU.
Biosci Rep. 2018 Jul 2;38(4):BSR20180005.
C57BL mice
B16 melanoma model
Intraperitoneal injection
35 mg/kg BCNU + 2.5×10^5 U/kg TNF
Single dose BCNU (day 0), TNF once daily for 5 days
To assess growth delay of B16 melanoma with combination therapy. Results showed significant prolongation of tumor doubling time in the combination group compared to monotherapy (P=0.005), without increased toxicity (weight loss or blood counts).
Br J Cancer. 1990 Nov;62(5):776-80.
C57BlC mice
GL261 glioblastoma model
Intraperitoneal injection
1 mg/kg，2.5 mg/kg,
24 hours
Test the therapeutic effect of RTV combined with BCNU on the GL261 glioblastoma model, found that the combination significantly prolonged mouse survival, and there was no significant difference between 1 mg/kg and 2.5 mg/kg BCNU
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