There will be a HazMat fee per item when shipping a dangerous goods. The HazMat fee will be charged to your UPS/DHL/FedEx collect account or added to the invoice unless the package is shipped via Ground service. Ship by air in Excepted Quantity (each bottle), which is up to 1g/1mL for class 6.1 packing group I or II, and up to 25g/25ml for all other HazMat items.
Type
HazMat fee for 500 gram (Estimated)
Excepted Quantity
USD 0.00
Limited Quantity
USD 15-60
Inaccessible (Haz class 6.1), Domestic
USD 80+
Inaccessible (Haz class 6.1), International
USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic
USD 100+
Accessible (Haz class 3, 4, 5 or 8), International
Bleomycin Sulfate is a glycopeptide antitumor antibiotic with antineoplastic efficacy[1]. Bleomycin sulfate treatment (3.5-4.0 mg/kg; intra-tracheal) on day 0, body weights decrease by day 4 then increases by Day 7 through the end of the study. Intra-tracheal bleomycin produced a statistically significant increase in lung hydroxyproline levels as well as increase in right caudal lung lobe mass[2]. Bleomycin sulfate (intratracheally; 2.5 mg/kg; 1.25 mg/ml, approximately 50 µl per mouse) induces pulmonary fibrosis in male C57BL/6 mice (8 weeks old, average weight approximately 24.5 g) [3].
体外研究
Bleomycin sulfate (BLM) has been selected as the most well-studied micronucleus (MN) inducer in human lymphocytes with different mechanisms of genotoxicity. The most common types of Bleomycin-induced DNA damage are single and double strand breaks and single depyrimidine/dehydropyrimidine sites. At the same time Bleomycin is a true radiomimetic compound, almost exactly resembling the genetic effects of ionising radiation [4].
The IC50 value of Bleomycin sulfate against the UT-SCC-19A cell line was 4.0±1.3 nM. Both UT-SCC-12A and UT-SCC-12B were more resistant to Bleomycin sulfate (BLM); IC50 values were 14.2±2.8 nM and 13.0±1.1 nM, respectively [5].
Bleomycin sulfate (50, 100 μM; 24, 48 h) induced pulmonary fibrosis in RLE-6TN cells (50 μM) and A549 cells (100 μM) [6].
Bleomycin Sulfate/硫酸博来霉素 细胞实验
Cell Line
Concentration
Treated Time
Description
References
Chlamydomonas reinhardtii
15 μg/ml
Used for screening transgenic lines.
Elife. 2021 Feb 15;10:e59119.
Non-IPF human lung fibroblasts
25 µg/ml
72 h
Induced UCP2 expression
Aging Cell. 2022 Sep;21(9):e13674.
293T cells
10 µM
16 h
To study the effect of Bleomycin on chromatin
Nucleic Acids Res. 2019 Jan 25;47(2):729-746.
MCF7 cells
50 µg/ml
1 h
To detect the DNA damage effect of Bleomycin on MCF7 cells. The results showed that Bleomycin treatment caused DNA double-strand breaks, and the DNA damage repair ability was reduced in Pol β-depleted cells.
Nucleic Acids Res. 2018 Jan 9;46(1):242-255.
U2OS cells
50 µg/ml
1 h
To detect the DNA damage effect of Bleomycin on U2OS cells. The results showed that Bleomycin treatment caused DNA double-strand breaks, and the DNA damage repair ability was reduced in Pol β-depleted cells.
Nucleic Acids Res. 2018 Jan 9;46(1):242-255.
Human fetal lung fibroblasts (HFL-1)
0.1 mU/ml
24, 48, 72 h
To investigate the effect of Bleomycin on HFL-1 cell proliferation, results showed that Bleomycin significantly increased the proliferation rate.
Respir Res. 2011 Nov 29;12(1):154.
Bleomycin Sulfate/硫酸博来霉素 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
C57BL/6 mice
Pulmonary fibrosis model
Intratracheal instillation
2.5 mg/kg
Single injection, observed at 7, 14, 21, and 28 days
To study the relationship between the development of pulmonary fibrosis and macrophage polarization, results showed that BLM-induced pulmonary fibrosis is closely related to M2 macrophage polarization.
J Nanobiotechnology. 2022 May 6;20(1):213
Mice
Bleomycin-induced lung fibrosis model
Oropharyngeal instillation
1.5 U/kg
Single dose, lasting 3 weeks
Studied the Bleomycin-induced lung fibrosis model, observed changes in UCP2 expression
Aging Cell. 2022 Sep;21(9):e13674.
C57BL/6 mice
Bleomycin-induced skin fibrosis model
Intradermal injection
1 mg/mL
Five times weekly for 3 weeks
The bleomycin-induced skin fibrosis model showed increased expression of WNT5A and EMT markers, consistent with human keloid tissue
Burns Trauma. 2022 Oct 7;10:tkac023.
C57BL/6 mice
Bleomycin-induced pulmonary fibrosis model
Subcutaneous injection
0.5 and 2.5 mg/kg
Once daily for 21 days
To evaluate the therapeutic effect of DR3penA on bleomycin-induced pulmonary fibrosis, the results showed that DR3penA significantly alleviated pulmonary fibrosis pathological damage and collagen deposition.
Acta Pharm Sin B. 2023 Feb;13(2):722-738.
C57BL/6 mice
Bleomycin-induced pulmonary fibrosis model
Intratracheal injection
5 mg/kg
Every three days for 21 days
Investigate macrophage polarization and pulmonary fibrosis development in bleomycin-induced pulmonary fibrosis model
Cell Commun Signal. 2018 Nov 23;16(1):89.
C57BL/6 mice
Bleomycin-induced lung fibrosis model
Intratracheal injection
0.025 U/mouse
Single dose, observed at 1, 2, 4 weeks
To investigate collagen deposition and inflammatory response in Bleomycin-induced lung fibrosis model, results showed that Bleomycin significantly increased collagen deposition in lung tissue.
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