There will be a HazMat fee per item when shipping a dangerous goods. The HazMat fee will be charged to your UPS/DHL/FedEx collect account or added to the invoice unless the package is shipped via Ground service. Ship by air in Excepted Quantity (each bottle), which is up to 1g/1mL for class 6.1 packing group I or II, and up to 25g/25ml for all other HazMat items.
Type
HazMat fee for 500 gram (Estimated)
Excepted Quantity
USD 0.00
Limited Quantity
USD 15-60
Inaccessible (Haz class 6.1), Domestic
USD 80+
Inaccessible (Haz class 6.1), International
USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic
USD 100+
Accessible (Haz class 3, 4, 5 or 8), International
5-Fluorouracil (5-FU), or combined with other chemotherapeutic agents, is widely used in the treatment of a range of cancers, including colorectal and breast cancers, head and neck cancers, cancers of the aerodigestive tract[1]. 5-Fluorouracil can disrupt RNA synthesis through RNA mis-incorporation by its active metabolites, as well as block dTMP synthesis through inhibition of thymidylate synthase. After entering into cells, 5-Fluorouracil can be converted to its active metabolites, fluorodeoxyuridine monophosphate (FdUMP), fluorodeoxyuridine triphosphate (FdUTP) and fluorouridine triphosphate[2]. FdUMP can inhibit the thymidylate synthase through binding to the nucleotide-binding site of thymidylate synthase, thus blocking the binding of normal substrate dUMP and inhibiting dTMP synthesis. And this will cause the disruption of DNA replication and repair[3]. FUTP can extensively incorporate into RNA and disrupt RNA processing and function[4]. Thus, 5-Fluorouracil showed cytotoxicity in various cells.
作用机制
The mechanism of cytotoxicity of 5-FU has been ascribed to the mis-incorporation of fluoronucleotides into RNA and DNA, as well as the inhibition of thymidylate synthase[4].
5-Fluorouracil/氟尿嘧啶 细胞实验
Cell Line
Concentration
Treated Time
Description
References
WiDr cells
0–115.2 μM
48 hours
To test the effect of SQLE knockdown on 5-FU sensitivity, results showed that SQLE knockdown increased the sensitivity of tumor cells to 5-FU treatment.
Cell Commun Signal. 2024 May 18;22(1):278.
RKO cells
0–115.2 μM
48 hours
To test the effect of SQLE knockdown on 5-FU sensitivity, results showed that SQLE knockdown increased the sensitivity of tumor cells to 5-FU treatment.
Cell Commun Signal. 2024 May 18;22(1):278.
Hct116 cells
20 ×10−6M
24 hours
To evaluate the effect of 5-FU on Hct116 cell growth, showing remarkable dose-dependent growth inhibition.
Adv Sci (Weinh). 2022 Oct;9(30):e2200717.
Detroit562
0.5–50 µM
72 h
To evaluate the resistance of CD10-positive subpopulation to 5-Fluorouracil, the results showed that the CD10-positive subpopulation was significantly more refractory to 5-Fluorouracil than the CD10-negative subpopulation.
Br J Cancer. 2014 Jul 29;111(3):506-14.
HUVECs
1 µg/mL
48 h
5-FU was used as a functional inhibitor of endothelial cells, cocultured with PD-MSCs and WKYMVm to evaluate its effect on endothelial cell activity and angiogenesis.
Cells. 2022 Jan 11;11(2):232.
MCF-7 cells
1, 2, 3, 4, 5, 6 µg/mL
24 h
To detect the sensitivity of MCF-7 cells to 5-fluorouracil, the results showed that EVs-treated cells had reduced sensitivity to 5-fluorouracil and significantly increased IC50.
Cell Biosci. 2021 Apr 5;11(1):68.
Yeast cells (Saccharomyces cerevisiae)
5 μg/ml
5 h
To investigate the effect of 5-FU on tRNAValAAC stability in trm4 trm8 double mutants. Results showed that 5-FU significantly reduced tRNAValAAC levels even at 30°C, and this effect was suppressed in met22 or xrn1 mutants.
Nucleic Acids Res. 2024 Jun 10;52(10):5841-5851
Yeast cells (Saccharomyces cerevisiae)
10 μg/ml
5 h
To investigate the effect of 5-FU on tRNAValAAC stability in yeast cells lacking specific tRNA methylations. Results showed that 5-FU significantly reduced tRNAValAAC levels in trm8 mutants, and this effect was suppressed in met22 or xrn1 mutants.
Nucleic Acids Res. 2024 Jun 10;52(10):5841-5851
5-Fluorouracil/氟尿嘧啶 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Mice
Wild-type, Hoxa9-deficient, and miR-155-deficient mice
Intraperitoneal injection
150 mg/kg
Single injection, lasting 4 days
To study the function of HOXA9 in bone marrow cells
Nucleic Acids Res. 2010 Sep;38(16):5472-8
Mice
APCmin/þ mouse model
Intravenous injection
50 mg/kg
Once daily for 8 weeks
To evaluate the anti-tumor effect of 5-FU in APCmin/t mice, the results showed that 5-FU significantly reduced the number and size of adenomas.
Nat Commun. 2017 Jan 4;8:14058
Mice
Primary myelofibrosis model
Intravenous injection
150 mg/kg
Single injection
Used to pre-treat donor mice for bone marrow cell collection
Nat Cell Biol. 2017 Jun;19(6):677-688
Mice
Chronic Myeloid Leukemia Model
200 mg/kg
Single dose
To construct a chronic myeloid leukemia model for studying the role of RalA in leukemia.
Int J Biol Sci. 2023 Feb 13;19(4):1211-1227
5-Fluorouracil/氟尿嘧啶 动物研究
Dose
Mice (i.p.): min = 10 mg/kg[5], max = 200 mg/kg[6]
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