alpha-Hederin (α-hederin), a monodesmosidic triterpenoid saponin, exhibited promising antitumor potential against a variety of human cancer cell lines. α-hederin significantly inhibited the proliferation of gastric cancer cells and arrested the cell cycle in G1 phase in vitro. α-hederin could inhibit the proliferation and induce apoptosis of gastric cancer accompanied by glutathione decrement and reactive oxygen species generation via activating mitochondrial dependent pathway[3]. α-Hederin could inhibit the proliferation and induce apoptosis of ESCC (esophageal squamous cell carcinoma) via dissipation of the MMP (mitochondrial membrane potential) with simultaneous ROS (reactive oxygen species) generation and activation of the mitochondrial pathway[4]. α‑hederin triggered apoptosis through ROS‑activated mitochondrial signaling pathway and autophagic cell death through ROS dependent AMPK(AMP‑activated protein kinase)/mTOR (mechanistic target of rapamycin) signaling pathway activation in colorectal cancer cells[5]. α-hederin suppressed IL-6-induced EMT (Epithelial-mesenchymal transition) associated with disruption of JAK2/STAT3 signaling in colon cancer cells[6].
α-Hederin/α－常春藤皂苷 细胞实验
Cell Line
Concentration
Treated Time
Description
References
HCT116 cells
10 µM
6, 12, 24 h
To evaluate the effect of α-Hederin on autophagy in HCT116 cells, results showed that α-Hederin activated the AMPK/mTOR signaling pathway to induce autophagy.
Int J Oncol. 2019 May;54(5):1601-1612.
HCT8 cells
0, 10, 60 µM
24 h
To evaluate the effect of α-Hederin on apoptosis in HCT8 cells, results showed that α-Hederin induced apoptosis in a dose-dependent manner.
Int J Oncol. 2019 May;54(5):1601-1612.
HCT116 cells
0, 10, 60 µM
24 h
To evaluate the effect of α-Hederin on apoptosis in HCT116 cells, results showed that α-Hederin induced apoptosis in a dose-dependent manner.
Int J Oncol. 2019 May;54(5):1601-1612.
HCT8 cells
0, 2, 4, 8, 16, 32, 64 µM
24, 48, 72 h
To evaluate the effect of α-Hederin on the proliferation of colorectal cancer cells, results showed that α-Hederin inhibited the viability of HCT8 cells in a dose- and time-dependent manner.
Int J Oncol. 2019 May;54(5):1601-1612.
HCT116 cells
0, 2, 4, 8, 16, 32, 64 µM
24, 48, 72 h
To evaluate the effect of α-Hederin on the proliferation of colorectal cancer cells, results showed that α-Hederin inhibited the viability of HCT116 cells in a dose- and time-dependent manner.
Int J Oncol. 2019 May;54(5):1601-1612.
NCI-H292 cells
18.04 μM (IC50)
48 h
To evaluate the antiproliferative effect of α-Hederin on NCI-H292 cells, results showed that α-Hederin significantly inhibited NCI-H292 cell proliferation.
Pharm Biol. 2021 Dec;59(1):11-20.
NCI-H460 cells
17.57 μM (IC50)
48 h
To evaluate the antiproliferative effect of α-Hederin on NCI-H460 cells, results showed that α-Hederin significantly inhibited NCI-H460 cell proliferation.
Pharm Biol. 2021 Dec;59(1):11-20.
A549 cells
13.75 μM (IC50)
48 h
To evaluate the antiproliferative effect of α-Hederin on A549 cells, results showed that α-Hederin significantly inhibited A549 cell proliferation.
Pharm Biol. 2021 Dec;59(1):11-20.
HaCaT cells
1–70 μg/mL
24 h
To evaluate the effect of α-Hederin on the viability of HaCaT cells, showing an IC50 value of 2.71±0.35 μg/mL
Molecules. 2019 Aug 15;24(16):2958.
SKOV-3 cells
1–70 μg/mL
24 h
To evaluate the effect of α-Hederin on the viability of SKOV-3 cells, showing an IC50 value of 2.62±0.04 μg/mL
Molecules. 2019 Aug 15;24(16):2958.
NCI-H1650 cells
12.5 μM
24 h
α-Hed blocked late autophagic flux in NSCLC cells by altering lysosomal pH and inhibiting lysosomal cathepsin D maturation, leading to autophagosome accumulation.
Int J Mol Sci. 2018 Oct 18;19(10):3221.
NCI-H1299 cells
12.5 μM
24 h
α-Hed blocked late autophagic flux in NSCLC cells by altering lysosomal pH and inhibiting lysosomal cathepsin D maturation, leading to autophagosome accumulation.
Int J Mol Sci. 2018 Oct 18;19(10):3221.
α-Hederin/α－常春藤皂苷 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Nude mice
HCT116 subcutaneous xenograft model
Intraperitoneal injection
2 mg/kg
Every 3 days for 3 weeks
To evaluate the effect of α-Hederin on autophagy in colorectal cancer cells in vivo, results showed that α-Hederin significantly inhibited tumor growth and promoted autophagy.
Int J Oncol. 2019 May;54(5):1601-1612.
BALB/c nude mice
A549 xenograft tumour model
Intraperitoneal injection
5 and 10 mg/kg
Every other day for 32 days
To evaluate the antitumor effect of α-Hederin on A549 xenograft tumours, results showed that α-Hederin significantly inhibited tumour growth.
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