iCRT14 has the capability to disrupt TCF binding to DNA, expanding its function beyond influencing TCF-β-cat interaction[1]. In BT-549 cells, iCRT14 administration (10, 25, 50 μM) significantly reduces cell proliferation in a concentration- and time-specific manner, albeit with less potency compared to iCRT3[2].
体内研究
When administered intraperitoneally (i.p.) at 50 mg/kg, iCRT14 notably reduces CycD1 levels and tumor cell proliferation in HCT116 xenograft models[1].
体外研究
iCRT14 has the capability to disrupt TCF binding to DNA, expanding its function beyond influencing TCF-β-cat interaction[1].
In BT-549 cells, iCRT14 administration (10, 25, 50 μM) significantly reduces cell proliferation in a concentration- and time-specific manner, albeit with less potency compared to iCRT3[2].
iCRT 14 细胞实验
Cell Line
Concentration
Treated Time
Description
References
Human coronary artery endothelial cells (HCAECs)
25 µM
18 hours
To assess the effect of iCRT-14 on TNF-α-stimulated endothelial cells. Results showed that iCRT-14 suppressed TNF-α-induced monocyte adhesion and decreased VCAM-1 protein levels.
Front Cardiovasc Med. 2023 Jan 17;9:1059124.
Human umbilical vein endothelial cells (HUVECs)
25 µM
18 hours
To evaluate the potential of iCRT-14 in reversing the adverse effects of TNF-α on endothelial physiology. Results showed that iCRT-14 lowered nuclear and total NFκB protein levels, as well as expression of NFκB target genes, IL-8 and MCP-1.
Front Cardiovasc Med. 2023 Jan 17;9:1059124.
HCC-1937
10, 25, 50 µM
48 hours
To evaluate the inhibitory effect of iCRT-14 on the proliferation of HCC-1937 cells. Results showed that iCRT-14 inhibited cell proliferation in a dose- and time-dependent manner.
J Transl Med. 2013 Nov 4;11:280.
HCC-1143
10, 25, 50 µM
48 hours
To evaluate the inhibitory effect of iCRT-14 on the proliferation of HCC-1143 cells. Results showed that iCRT-14 inhibited cell proliferation in a dose- and time-dependent manner.
J Transl Med. 2013 Nov 4;11:280.
MDA-MB-231
10, 25, 50 µM
48 hours
To evaluate the inhibitory effect of iCRT-14 on the proliferation of MDA-MB-231 cells. Results showed that iCRT-14 inhibited cell proliferation in a dose- and time-dependent manner.
J Transl Med. 2013 Nov 4;11:280.
BT-549
10, 25, 50 µM
48 hours
To evaluate the inhibitory effect of iCRT-14 on the proliferation of BT-549 cells. Results showed that iCRT-14 inhibited cell proliferation in a dose- and time-dependent manner.
J Transl Med. 2013 Nov 4;11:280.
ST2 cells
10 µM
72 hours
To verify the specificity of Wnt signaling, ICRT-14 reversed the effects of S33 on the differentiation of ST2 cells
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