There will be a HazMat fee per item when shipping a dangerous goods. The HazMat fee will be charged to your UPS/DHL/FedEx collect account or added to the invoice unless the package is shipped via Ground service. Ship by air in Excepted Quantity (each bottle), which is up to 1g/1mL for class 6.1 packing group I or II, and up to 25g/25ml for all other HazMat items.
Type
HazMat fee for 500 gram (Estimated)
Excepted Quantity
USD 0.00
Limited Quantity
USD 15-60
Inaccessible (Haz class 6.1), Domestic
USD 80+
Inaccessible (Haz class 6.1), International
USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic
USD 100+
Accessible (Haz class 3, 4, 5 or 8), International
TTNPB (Ro 13-7410) (GMP) refers to TTNPB produced following GMP guidelines. GMP small molecules serve as appropriate auxiliary reagents for cell therapy manufacture. TTNPB is a highly potent retinoic acid receptor (RAR) agonist.
体外研究
TTNPB inhibits binding of [3H]tRA with IC50s of 3.8 nM, 4 nM, and 4.5 nM for human RARα, β, and γ, respectively. TTNPB competes for [3H]tRA binding to CRABPI with IC50s of 1800 nM[1].
The combination of TTNPB (Ro 13-7410) (GMP) (100 nM) and Laduviglusib can induce chondrogenic markers in hiPSCs and form hyaline cartilaginous tissues in vivo[2].
TTNPB/芳香维甲酸 细胞实验
Cell Line
Concentration
Treated Time
Description
References
H1 hESCs
3 nM
4 days
Induce differentiation of H1 hESCs into pancreatic endoderm
Nucleic Acids Res. 2018 Apr 6;46(6):2883-2900.
human pluripotent stem cells
6 µM
Used in mesoderm differentiation experiments, TTNPB was added to the medium to promote mesodermal cell differentiation.
Cells. 2025 Jan 24;14(3):184.
mouse primary brown adipocytes
100 nM
18 h
Pretreatment with TTNPB restored mitochondrial respiration in Rdh1-null brown adipocytes to WT levels and restored the expression of lipolysis-related genes to WT levels.
Cell Mol Life Sci. 2019 Jun;76(12):2425-2447.
Bovine ear fibroblasts
1 μM
6 days
Induce the transdifferentiation of bovine ear fibroblasts into adipocyte-like cells, with a large number of lipid droplets observed on day 6
Front Cell Dev Biol. 2023 Feb 2;11:1020965.
M14(5–16) cells
1 μM
6 days
To evaluate the effect of TTNPB on the proliferation of M14(5–16) cells, results showed that TTNPB alone reduced cell proliferation by 22%, and in combination with LGD1069, it had an additive effect, reducing cell proliferation by 38%.
Mol Cancer. 2009 Mar 6;8:16.
L929 mouse fibroblast cell line
1 µM
8 days
Induced L929 cells to transform into dermal-papilla-cell-like cells (DPC-LCs), confirmed by alkaline phosphatase (ALP) staining and molecular markers
Int J Mol Sci. 2022 Apr 11;23(8):4213.
Primary adult mouse fibroblasts
1 µM
8 days
TTNPB significantly increased ALP activity in primary adult mouse fibroblasts and confirmed their transformation into DPC-LCs through molecular markers
Int J Mol Sci. 2022 Apr 11;23(8):4213.
Human embryonic stem cells (H9)
0.5 μM
4 days
TTNPB significantly improved the clonal colony formation efficiency of human embryonic stem cells, increasing it from 0-0.3% to 2-3% when used alone, and to 15-17% when combined with Y-27632.
Commun Biol. 2024 Feb 16;7(1):190.
Human induced pluripotent stem cells (4YF)
0.5 μM
4 days
TTNPB significantly improved the clonal colony formation efficiency of human induced pluripotent stem cells, increasing it from 0-0.3% to 2-3% when used alone, and to 15-17% when combined with Y-27632.
Commun Biol. 2024 Feb 16;7(1):190.
TTNPB/芳香维甲酸 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Nude mice
Full-thickness wound model in nude mice
Subcutaneous injection
1 µM
Single injection, lasting 3 weeks
Assessed the ability of TTNPB-treated fibroblasts to induce hair follicle regeneration in vivo, results showed TTNPB-treated cells could induce HF-like structure formation
Binding affinity against retinoic Acid gamma receptors co-transfected into CV-1 cells, EC50=0.002 Μm
8308867
HEK293 cells
Function assay
Agonist activity at human RARalpha expressed in HEK293 cells by luciferase reporter gene assay, EC50=0.18 nM
25305688
HeLa cells
Function assay
Agonist activity at human RARalpha expressed in human HeLa cells assessed as relative luminescence units at >=10 uM by luciferase assay relative to control
18951029
HL60 cells
Cytotoxicity assay
In vitro cytotoxicity against HL60 cells, IC50=46 μM
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