TTK21 is an activator of the histone acetyltransferases CBP/p300. TTK21 crosses the blood-brain barrier, is non-toxic, and reaches different parts of the brain when bound to glucose-based carbon nanospheres (CSPs). TTK21 has shown beneficial effects on brain functions such as neurogenesis and long-term memory in adults[1].
TTK21 细胞实验
Cell Line
Concentration
Treated Time
Description
References
HeLa cells
50, 100, 200, 275 µM
24 hours
To examine the effect of TTK21 on histone H3 acetylation, results showed no significant alteration of histone H3 acetylation in response to TTK21 treatment
J Neurosci. 2013 Jun 26;33(26):10698-712
Rat hippocampal slices
0.36 μg/ml CSP-TTK21
30 min
To study the rescue effect of CSP-TTK21 on Aβ(1-42)-induced synaptic plasticity deficits, results showed CSP-TTK21 restored Aβ(1-42)-induced LTP deficits
Aging Cell. 2022 Sep;21(9):e13675
SHSY-5Y neural cells
50 μg/ml
6, 12, 24 hours
To examine the effect of CSP-TTK21 on histone H3K14 acetylation, results showed a significant increase in H3K14 acetylation levels
J Neurosci. 2013 Jun 26;33(26):10698-712
TTK21 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Adult male Wistar rats
Aβ(1-42)-induced AD model
Bath application to hippocampal slices
0.36 μg/mL
30 min before and 30 min after LTP induction
To study the rescue effect of CSP-TTK21 on Aβ(1-42)-induced synaptic plasticity deficits, results showed CSP-TTK21 restored synaptic plasticity and synaptic tagging and capture (STC)
Aging Cell. 2022 Sep;21(9):e13675
Mice
Adult mice
Intraperitoneal injection
20 mg/kg
Single injection
To examine the effect of CSP-TTK21 on histone acetylation in the mouse brain, results showed a significant increase in histone acetylation levels in the hippocampus and frontal cortex, and promoted the maturation and differentiation of adult neurons
J Neurosci. 2013 Jun 26;33(26):10698-712
Mice
Syngap1+/− mouse model
Oral
20 mg/kg
Once per week for 3 weeks
Restoration of synaptic functions and improvement of behavioral deficits
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