There will be a HazMat fee per item when shipping a dangerous goods. The HazMat fee will be charged to your UPS/DHL/FedEx collect account or added to the invoice unless the package is shipped via Ground service. Ship by air in Excepted Quantity (each bottle), which is up to 1g/1mL for class 6.1 packing group I or II, and up to 25g/25ml for all other HazMat items.
Type
HazMat fee for 500 gram (Estimated)
Excepted Quantity
USD 0.00
Limited Quantity
USD 15-60
Inaccessible (Haz class 6.1), Domestic
USD 80+
Inaccessible (Haz class 6.1), International
USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic
USD 100+
Accessible (Haz class 3, 4, 5 or 8), International
The soluble epoxide hydrolase (sEH) converts epoxides to diols by catalytic addition of a water molecule. It has been shown to metabolize fatty acid epoxides in various human diseases. TPPU is a potent sEH inhibitor with IC50 values of 3.7 and 2.8nM for human and murine sHE, respectively[1]. TPPU also shows inhibitory activity against monkey sEH with an IC50 value of 37nM[2]. TPPU binds to recombinant human sEH with a Ki value of 0.9±0.1nM and koff of 10.5×10-4/s. Administration of rats with 10mg/kg TPPU via oral gavage inhibited human sEH with an IC50 value of 1.1±0.1nM[3].
TPPU reduced the number of BLM-induced β-gal positive cells.
Redox Biol. 2023 Jul;63:102765
Mixed mouse glial cultures
10 µM
30 minutes
To evaluate the effect of TPPU on mixed glial inflammation, results showed that TPPU suppressed microglial reactivity through astrocytes.
Sci Transl Med. 2020 Dec 9;12(573):eabb1206
Mouse primary microglia
10 µM
30 minutes
To evaluate the effect of TPPU on microglial inflammation, results showed that TPPU had no direct effect on microglia.
Sci Transl Med. 2020 Dec 9;12(573):eabb1206
Mouse primary astrocytes
0.5 µM to 10 µM
30 minutes
To evaluate the effect of TPPU on LPS-induced inflammation, results showed that TPPU dose-dependently reduced nitrite release and pro-inflammatory molecule expression.
Sci Transl Med. 2020 Dec 9;12(573):eabb1206
MLE12 cells
20 µM
48 hours
TPPU significantly reduced the number of BLM-induced β-gal positive cells and reduced the expression of senescence-related p53 and p21 proteins in MLE12 cells.
Redox Biol. 2023 Jul;63:102765
TPPU 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
C57BL/6J mice
Bleomycin-induced pulmonary fibrosis model
Intraperitoneal injection
0.25 mg/kg
5 days per week for 3 weeks
TPPU significantly attenuated bleomycin-induced pulmonary fibrosis and reduced collagen levels
Exp Mol Med. 2021 May;53(5):864-874
Mice
Inflammation and social defeat stress models
Oral
0.3, 1.0, or 3.0 mg/kg
Single administration or continuous administration for 3 weeks
TPPU showed rapid antidepressant effects in both inflammation and social defeat stress models, preventing and alleviating depression-like behaviors.
Proc Natl Acad Sci U S A. 2016 Mar 29;113(13):E1944-52
Mice
MPTP-induced neurotoxicity model
Oral
0.3, 1.0, or 3.0 mg/kg
Twice daily from day 2 to day 7
TPPU significantly attenuated MPTP-induced dopaminergic neurotoxicity in the striatum and substantia nigra
Proc Natl Acad Sci U S A. 2018 Jun 19;115(25):E5815-E5823
C57BL/6J mice
D-galactose-induced premature aging mouse model
Intraperitoneal injection
1 mg/kg
Once daily for six weeks
TPPU significantly inhibited the protein expression of p16, p21, and γH2AX in D-gal-induced premature aging mice and reduced the degree of age-related pulmonary fibrosis.
Redox Biol. 2023 Jul;63:102765
Male C57BL/6N mice
Scald injury model
Intraperitoneal injection
10 mg/kg
Three administrations at 48 h, 24 h, and directly before burn injury
To evaluate the effect of TPPU on DiHOME levels and immune cell necrosis in scalded mice, it was found that TPPU significantly reduced 12,13-DiHOME levels and decreased necrosis of innate and adaptive immune cells in a dose-dependent manner.
Proc Natl Acad Sci U S A. 2022 Mar 29;119(13):e2120691119
Mice
Maternal immune activation (MIA) model
Drinking water
15 mg/L
From P28 to P56
TPPU treatment prevented cognitive deficits and the reduction of PV and GAD67 immunoreactivity in the medial prefrontal cortex (mPFC) of adult offspring after MIA.
Proc Natl Acad Sci U S A. 2019 Apr 2;116(14):7083-7088
C57BL/6J mice
Myocardial infarction model
Oral (via drinking water)
15 mg/L
3 weeks
To evaluate the effect of TPPU on cardiac function and fibrosis post-myocardial infarction, results showed that TPPU significantly improved cardiac function and reduced cardiac fibrosis.
Proc Natl Acad Sci U S A. 2013 Apr 2;110(14):5618-23
C57BL/6 mice
PM2.5-mediated lung injury model
Intragastric administration
3 mg/kg
Three times per week for three weeks
To evaluate the protective effect of TPPU on PM2.5-mediated lung injury. Results showed that TPPU treatment reversed PM2.5-induced pulmonary hyperemia, edema, alveolar wall thickening, and macrophage activation, reduced levels of TNF-α, IL-6, MPO, and LDH, and alleviated inflammatory responses by inhibiting the MAPK/NF-κB signaling pathway.
J Hazard Mater. 2023 Sep 15;458:131890
Mice
5xFAD transgenic mice
Oral gavage or drinking water
3 mg/kg
24 hours or 2.5 to 4.5 months
To evaluate the effect of TPPU on acute inflammation and long-term AD pathology, results showed that TPPU reduced neuroinflammation, improved Aβ pathology, and cognitive function.
Sci Transl Med. 2020 Dec 9;12(573):eabb1206
Mice
Destabilization of the medial meniscus (DMM) model
Intraperitoneal injection
3mg/kg
Single injection, measured at 3 hours post-injection
Acute TPPU injection significantly reversed DMM-induced pain behaviors, including increased weight-bearing on the ipsilateral hind-limb and reversal of the lowered ipsilateral hind-paw withdrawal thresholds.
搜索
HazMat Fee +
