Sinefungin is a potent inhibitor of virion mRNA(guanine-7-)-methyltransferase, mRNA(nucleoside-2'-)-methyltransferase and viral proliferation [1].Sinefungin is an SET7/9 inhibitor that ameliorates renal fibrosis by inhibiting H3K4 methylation[2].Treatment of cells with Sinefungin at concentrations of 0.5 or 1.0 μg/mL for 60 min ameliorated TGF-β1-induced increases in α-SMA and inhibited the up-regulation of histone H3K4 monomethylation in renal epithelial cells and renal fibroblasts[2].
Sinefungin 细胞实验
Cell Line
Concentration
Treated Time
Description
References
Vero cells
2.56 mM
20 hours
To evaluate the effect of SIN on replication of vesicular stomatitis virus(VSV), results showed that SIN significantly inhibited viral replication.
J Virol. 2007 Apr;81(8):4104-15
Saccharomyces cerevisiae
10 µM
24 hours
To study the inhibitory effect of Sinefungin on yeast growth, it was found that Sinefungin inhibits yeast growth by inhibiting the function of the AdoMet transporter Sam3.
Nucleic Acids Res. 2007;35(20):6895-903
BHK cells
2.56 mM
24 hours
To evaluate the effect of SIN on replication of vesicular stomatitis virus(VSV), results showed that SIN significantly inhibited viral replication.
J Virol. 2007 Apr;81(8):4104-15
Sinefungin 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Sprague-Dawley rats
Immunosuppressed rat model
Oral
0.01 to 10.0 mg/kg/day
Administered from day 7 to day 21
Sinefungin demonstrated curative and preventive activity against C. parvum infection in the immunosuppressed rat model, with dose-related suppression of oocyst shedding correlated with oocyst disappearance from ileal sections.
Daily administration of Sinefungin at a dose of 10 mg/kg to animals after induction of the Unilateral Ureteral Obstruction model improved renal fibrosis in obstructive nephropathy[2].
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