There will be a HazMat fee per item when shipping a dangerous goods. The HazMat fee will be charged to your UPS/DHL/FedEx collect account or added to the invoice unless the package is shipped via Ground service. Ship by air in Excepted Quantity (each bottle), which is up to 1g/1mL for class 6.1 packing group I or II, and up to 25g/25ml for all other HazMat items.
Type
HazMat fee for 500 gram (Estimated)
Excepted Quantity
USD 0.00
Limited Quantity
USD 15-60
Inaccessible (Haz class 6.1), Domestic
USD 80+
Inaccessible (Haz class 6.1), International
USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic
USD 100+
Accessible (Haz class 3, 4, 5 or 8), International
Schizandrin is a major bioactive constituent of Schisandra chinensis (Turcz.) Baill with antioxidant and anti-inflammatory properties. Schizandrin prevented LPS-induced injury in the H9c2 cells and promoted the recovery of myocardial tissues by enhancing cell viability and migration[3]. Sch A specifically reverses P-gp-mediated DOX resistance in MCF-7/DOX cells by blocking P-gp, NF-κB, and Stat3 signaling. Inhibition of P65 and Stat3 shows potent anti-MDR (Multidrug resistance) effect on MCF-7/DOX cells[4]. Schizandrin (1 and 10 mg/kg, p.o.) significantly reversed the scopolamine-induced impairment of spatial memory. Similarly, schizandrin (1 mg/kg, p.o.) significantly reversed the scopolamine-induced impairment of the passive avoidance response. Moreover, in mice, schizandrin (1 and 10 mg/kg, p.o.) enhanced tremors induced by oxotremorine, a muscarinic M(1) receptor agonist[5]. Pretreatment of the cortical cell cultures with schizandrin (10, 100 microM) for 2 h significantly protected cortical neurons against Glu-induced excitotoxicity. The neuroprotective activity of schizandrin was the most potent at the concentration of 100 microM. In addition, schizandrin diminished the intracellular Ca2+ influx, inhibited the subsequent overproduction of nitric oxide (NO), reactive oxygen species (ROS), and cytochrome c, and preserved the mitochondrial membrane potential. Furthermore, schizandrin also increased the cellular level of glutathione (GSH) and inhibited the membrane lipid peroxidation malondialdehyde (MDA)[6]. Schizandrin can be used to treat inflammatory and atopic diseases through the inhibition of TSLP(Thymic stromal lymphopoietin) [7].
Schisandrin/五味子醇甲 细胞实验
Cell Line
Concentration
Treated Time
Description
References
Primary cortical neurons
1 µM
18 days
Promote neuronal maturation and axon growth
Molecules. 2021 Dec 9;26(24):7466
RAW 264.7 macrophages
50, 100, 200 µM
1 hour pretreatment followed by 24 hours LPS stimulation
Inhibited LPS-induced NO and PGE2 production, downregulated iNOS and COX-2 mRNA and protein expression
Int J Mol Med. 2018 Jan;41(1):264-274
Bone marrow-derived macrophages (BMMs)
200 µM
2 days
To evaluate the effect of Schisandrin A on BMMs apoptosis, results showed 200 μmol/L concentration did not induce apoptosis
Cell Prolif. 2020 Oct;53(10):e12882.
BEAS‑2B
0, 25, 50, 75, 100 µM
24 hours
SchA reduced the viability of BEAS‑2B cells in a concentration-dependent manner.
Int J Mol Med. 2021 Dec;48(6):214.
H1299
0, 25, 50, 75, 100 µM
24 hours
SchA reduced the viability of H1299 cells in a concentration-dependent manner.
Int J Mol Med. 2021 Dec;48(6):214.
H1975
0, 25, 50, 75, 100 µM
24 hours
SchA reduced the viability of H1975 cells in a concentration-dependent manner.
Int J Mol Med. 2021 Dec;48(6):214.
A549
0, 25, 50, 75, 100 µM
24 hours
SchA reduced the viability of A549 cells in a concentration-dependent manner.
Int J Mol Med. 2021 Dec;48(6):214.
Rat chondrocytes
25, 50 µM
24 hours
To evaluate the effects of Schisandrin A on IL-1β-induced inflammation and cartilage degradation. Results showed that Schisandrin A could suppress IL-1β-induced production of NO and PGE2, downregulate iNOS and COX2 expression, inhibit MMPs and ADAMTS5 expression, and reverse IL-1β-induced downregulation of Collagen II, Aggrecan, and Sox9.
Front Pharmacol. 2019 Jan 29;10:41
Bone marrow-derived macrophages (BMMs)
0, 50, 100, 150, 200, 400 µM
24, 48, 72 hours
To evaluate the effect of Schisandrin A on BMMs cell viability, results showed no significant effect on cell viability below 200 μmol/L
Cell Prolif. 2020 Oct;53(10):e12882.
THP-1 cells
20/40/80 µM
4 hours
To investigate the effect of SCH on NLRP3 inflammasome activation in THP-1 cells, results showed that SCH could inhibit NLRP3 inflammasome activation.
Chin Med. 2023 Sep 6;18(1):112
Spd2 cells
5, 10, 20 mM
48 hours
To evaluate the effect of Schisandrin A on the viability of Spd2 cells. Results showed that Schisandrin A promoted the proliferation of testicular interstitial cells at concentrations of 10–40 mmol/L, with the best effect observed at 10 mmol/L.
Acta Pharm Sin B. 2023 Jun;13(6):2765-2777.
Rat colonic smooth muscle cells
0.5-10 µM
5 minutes
To investigate the inhibitory effect of Schisandrin on spontaneous contraction of rat colon, results showed that Schisandrin inhibited colonic spontaneous contraction in a concentration-dependent manner with an EC50 of 1.66 μM.
Phytomedicine. 2011 Aug 15;18(11):998-1005
Neural progenitor cells (NPCs)
1 µM
72 hours
Promote the proliferation of neural progenitor cells
Molecules. 2021 Dec 9;26(24):7466
Schisandrin/五味子醇甲 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
C57BL/6 mice
Ovariectomy-induced osteoporosis model
Injection
100 mg/kg
Every other day for 6 weeks
To evaluate the effect of Schisandrin A on ovariectomy-induced osteoporosis, results showed it significantly reduced bone loss
Cell Prolif. 2020 Oct;53(10):e12882.
C57/BL6 mice
Ischemic brain injury model
Intraperitoneal injection
12 mg/kg
Once daily for 3 weeks
Promote neural progenitor cell proliferation and nerve fiber regeneration
Molecules. 2021 Dec 9;26(24):7466
APP/PS1 transgenic mice
Alzheimer's disease model
Intragastrically
2 mg/kg/day
Once daily for 2 weeks
Schisandrin ameliorated learning and memory impairments in APP/PS1 mice and significantly decreased Aβ deposition in the hippocampus. Additionally, Schisandrin treatment restored the abnormal levels of neurotransmitters and their metabolites in the brain.
Acta Pharmacol Sin. 2018 Apr;39(4):616-625
C57BL/6 mice
DSS-induced ulcerative colitis model
Gavage
20, 40, 80 mg/kg/d
Once daily for 7 days
To evaluate the therapeutic effect of SCH on ulcerative colitis, results showed that SCH significantly alleviated colitis symptoms, reduced inflammatory factors, and restored gut microbiota balance.
Chin Med. 2023 Sep 6;18(1):112
Sprague-Dawley rats
OA model induced by anterior cruciate ligament transection and partial medial meniscectomy
Intra-articular injection
50 μM
Once weekly for one month
To evaluate the protective effects of Schisandrin A on cartilage damage in OA model. Results showed that Schisandrin A significantly alleviated joint damage and reduced cartilage destruction.
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