Saikosaponin D, a natural product isolated and purified from the herb of Bupleurum chinense DC., is a SERCA inhibitor by inhibiting NF-κB and STAT3 signaling to protect against acetaminophen-induced hepatotoxicity and a potent inhibitor on acute hepatic injury by CCl4 and a potent cytotoxicity agent for human hepatocellular carcinoma cells, and has the antiproliferative effect in A549 cells that may be the induction of p53 and activity of the Fas/FasL apoptotic system.
Saikosaponin D/柴胡皂苷D 细胞实验
Cell Line
Concentration
Treated Time
Description
References
Hippocampal neural progenitor cells (NPCs)
5.13 µM (EC50)
24 hours
SSd inhibited the survival and proliferation of NPCs and induced apoptosis by activating the NRAGE/NADE/NRIF apoptotic signaling pathway.
Clin Transl Med. 2020 Dec;10(8):e243
HepG2 cells
7.5-15 µM
24 hours
Induced autophagy and apoptosis, detected by GFP-LC3 puncta formation and Annexin V staining.
Cell Death Dis. 2013 Jul 11;4(7):e720
HepG2 cells
7.5-15 µM
24 hours
Induced autophagy and apoptosis, confirmed by GFP-LC3 puncta formation and Annexin V staining.
Cell Death Dis. 2013 Jul 11;4(7):e720
MCF-7 cells
10 µM
4 hours
Induced autophagy, detected by GFP-LC3 puncta formation and increased LC3-II protein levels.
Cell Death Dis. 2013 Jul 11;4(7):e720
HeLa cells
10 µM
4 hours
Induced autophagy, detected by GFP-LC3 puncta formation and increased LC3-II protein levels.
Cell Death Dis. 2013 Jul 11;4(7):e720
MCF-7 cells
10 µM
4 hours
Induced autophagy, confirmed by GFP-LC3 puncta formation and increased LC3-II protein expression.
Cell Death Dis. 2013 Jul 11;4(7):e720
HeLa cells
10 µM
4 hours
Induced autophagy, confirmed by GFP-LC3 puncta formation and increased LC3-II protein expression.
Cell Death Dis. 2013 Jul 11;4(7):e720
U937 cells
1 µM
48 hours
Inhibited cell proliferation, promoted apoptosis and cell-cycle arrest
Theranostics. 2021 Mar 31;11(12):5831-5846
MV4-11 cells
1 µM
48 hours
Inhibited cell proliferation, promoted apoptosis and cell-cycle arrest
Theranostics. 2021 Mar 31;11(12):5831-5846
Kasumi-1 cells
1 µM
48 hours
Inhibited cell proliferation, promoted apoptosis and cell-cycle arrest
Theranostics. 2021 Mar 31;11(12):5831-5846
NB4 cells
1 µM
48 hours
Inhibited cell proliferation, promoted apoptosis and cell-cycle arrest
Theranostics. 2021 Mar 31;11(12):5831-5846
HeLa cells
15 µM
6 hours
To assess the ability of SsD to modulate autophagy, it was found that SsD significantly inhibited the fusion of autophagosomes and lysosomes, leading to the accumulation of autophagosomes, increased lysosomal pH, and TFEB nuclear translocation.
Signal Transduct Target Ther. 2019 Feb 22;4:4
Saikosaponin D/柴胡皂苷D 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
C57BL/6N mice
Leukemia model
Intraperitoneal injection
0.1 mg/kg or 0.5 mg/kg
3 times per week for 3 weeks
Significantly inhibited leukemia progression, including reduced WBC count, decreased leukemic blasts in BM, reduced splenomegaly, inhibited lung metastasis and prolonged survival
Theranostics. 2021 Mar 31;11(12):5831-5846
C57BL/6J mice
Cognitive dysfunction model
Oral gavage
16 mg/kg
Once daily for 14 consecutive days
SSd inhibited hippocampal neurogenesis and caused cognitive dysfunction by disrupting pro-BDNF/p75NTR and BDNF/TrkB signaling pathways.
Clin Transl Med. 2020 Dec;10(8):e243
Mice
Experimental autoimmune encephalomyelitis (EAE) model
Intragastric administration
40 mg/kg
Once daily, starting from the day of immunization
To investigate the therapeutic effect of SSD on EAE, results showed that SSD significantly alleviated EAE symptoms
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