S0859, an N-cyanosulphonamide compound, reversibly inhibit NBC-mediated pH (i) recovery (K (i)=1.7 mM, full inhibition at approximately 30 mM).
S0859 细胞实验
Cell Line
Concentration
Treated Time
Description
References
A549 cells
20 µM
24 hours
To evaluate the effect of S0859 on cell migration, results showed that S0859 partially inhibited cell motility.
Pharmaceutics. 2020 Aug 27;12(9):816
Mouse hippocampal neurons
50 µM
30 minutes
To investigate the effect of S0859 on GABA A receptor-mediated postsynaptic currents. Results showed that S0859 completely inhibited postsynaptic GABA AR and, thus, could not be used as a tool to investigate the role of NBCs in GABA-dependent neuronal networks.
Front Cell Neurosci. 2023 Oct 10;17:1253424
HEK-293 cells
50 µM
30 minutes
To investigate the effect of NBC inhibitor S0859 on KCC2 activity. Results showed that S0859 prevented staurosporine- and 4-aminopyridine(4AP)-induced KCC2 activation.
Front Cell Neurosci. 2023 Oct 10;17:1253424
Mouse renal afferent arterioles
50 µM
30 minutes
S0859, an inhibitor of the NBC family, blocked the NaHCO3-induced increase in pH i and vasodilation
Hypertension. 2019 Nov;74(5):1104-1112
LNCaP cells
50 µM
4 days
To assess the role of NBCe1 in LNCaP cell proliferation and viability, results showed that S0859 treatment decreased the number of viable cells
Oncol Rep. 2021 Jul;46(1):129
Ciliated human nasal epithelial cells (c-hNECs)
30 µM
5 minutes
S0859 decreased the CBF ratio from 1.00 to 0.79, indicating that the NBC inhibitor reduced HCO3- influx, thereby lowering pH i and CBF.
Int J Mol Sci. 2024 Aug 21;25(16):9069
PC3 cells
100 µM
6 days
To assess the role of NBCe1 in PC3 cell proliferation and viability, results showed that S0859 treatment decreased the number of viable cells
Oncol Rep. 2021 Jul;46(1):129
Human keratinocytes (HaCaT)
100 µM
6 hours
To evaluate the inhibitory effect of S0859 on SLS-induced cytotoxicity in keratinocytes. Results showed that S0859 significantly inhibited SLS-dependent cell death.
Front Cell Infect Microbiol. 2022 Nov 1;12:1002230
To evaluate the inhibitory effect of S0859 on RA-FLSs migration. Results showed that S0859 significantly reduced TNF-α or RA synovial fluid-stimulated RA-FLSs migration.
Exp Mol Med. 2022 Apr;54(4):503-517
MCF-7 cells
50 µM
7 days
To evaluate the effect of S0859 on MCF-7 spheroid growth, results showed that S0859 combined with MCT1 inhibitor AR-C155858 further reduced spheroid diameter
Mol Cancer. 2016 Jun 6;15(1):45
S0859 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
C57BL/6 mice
Necrotizing skin infection model
Subcutaneous injection
200 µM
Initial injection followed by additional doses at 4 and 8 hours post-infection, lasting 24 hours
To evaluate the effect of S0859 on SLS-dependent skin lesions. Results showed that S0859 treatment did not significantly affect SLS-mediated skin lesion size.
Front Cell Infect Microbiol. 2022 Nov 1;12:1002230
DBA/1 mice
Collagen-induced arthritis (CIA) model
Tail vein injection
800 ng/g
Two injections, two weeks apart
To evaluate the inhibitory effect of S0859 on joint swelling and destruction in the CIA mouse model. Results showed that S0859 reduced joint swelling and destruction without blood, hepatic, or renal toxicity.
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