货号：A126336同义名：
维生素A酸; 全反式维甲酸
/ Vitamin A acid; all-trans-Retinoic acid
All-Trans Retinoic Acid (Vitamin A acid)作为 RAR 核受体的激动剂，用于细胞疗法中的辅助试剂。All-Trans Retinoic Acid促进小鼠和人类多能干细胞分化为运动神经元，促进神经干细胞的神经元分化，促进人类胚胎干细胞 (ES 细胞) 分化为胰腺祖细胞，促进小鼠 ES 细胞的脂肪细胞分化，促进小鼠 ES 细胞的心室心肌细胞分化，促进粒细胞的终末分化。
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Limited Quantity
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Retinoic acid receptors (RARs) are members of the super‐family of nuclear hormone receptors, which directly regulate the transcription of their target genes in the nucleus. Retinoic acid, the active metabolite of vitamin A, is a ligand for retinoic acid receptor (RAR) and retinoid X receptor (RXR). It has pleiotropic effects on growth, differentiation, proliferation, and development, and determines embryonic pattern formation and inhibits tumor growth. Addition of tretinoin significantly suppressed the remarkably elevated MMP-13 mRNA expression in the keloid‐derived dermal fibroblasts, with the peak suppression at 12h[3]. Tretinoin also prevented the cytotoxicity of H2O2 in cultured human mesangial cells by increasing both the catalase activity and the reduced glutathione content in dose- and time-dependent manner[4]. Retinoic acid at 10 μM inhibited the migration of Aspc-1 and Panc-1 cells indirectly through its inhibitory effects on cancer associated fibroblasts (CAFs) and inhibited epithelial-mesenchymal transition of tumor cells by decreasing IL-6 secretion of CAFs[5]. Neural stem cells (NSCs) treated with 1 µM retinoic acid for 4 days showed increased HRas and Tuj1 levels together with the increase in the active HRas level, which was required for NSC differentiation into neurons and murine brain development[6].
Retinoic acid/维A酸 细胞实验
Cell Line
Concentration
Treated Time
Description
References
HepG2
5 µM and 10 µM
48 hours
Enhanced the cytotoxicity of sorafenib
Cancer Sci. 2015 May;106(5):567-75
Human fetal lung capillary endothelial precursor cells
0.01, 0.1, 1 µM
24 hours
To evaluate the effect of RA on endothelial cell proliferation, results showed RA increased cell proliferation in a concentration-dependent manner.
Am J Respir Cell Mol Biol. 2005 Dec;33(6):622-8.
THP-1 cells
10 µM
24 hours
To evaluate the inhibitory effect of Palovarotene on HSV-1 replication, results showed RA inhibited HSV-1 replication in a dose-dependent manner.
iScience. 2024 Jun 4;27(7):110144
Hep3B
5 µM and 10 µM
48 hours
Enhanced the cytotoxicity of sorafenib
Cancer Sci. 2015 May;106(5):567-75
HLF
5 µM and 10 µM
48 hours
Enhanced the cytotoxicity of sorafenib
Cancer Sci. 2015 May;106(5):567-75
HLE
5 µM and 10 µM
48 hours
Enhanced the cytotoxicity of sorafenib
Cancer Sci. 2015 May;106(5):567-75
HuH6
5 µM and 10 µM
48 hours
Enhanced the cytotoxicity of sorafenib
Cancer Sci. 2015 May;106(5):567-75
PLC/PRF/5
5 µM and 10 µM
48 hours
Enhanced the cytotoxicity of sorafenib
Cancer Sci. 2015 May;106(5):567-75
neurons
2 μM
1 week
to induce neuronal differentiation
Nat Biotechnol. 2011 Aug 10;29(9):824-8.
GABAergic neurons
1 μM
7 days
to induce GABAergic neuron differentiation
Nat Biotechnol. 2011 Aug 10;29(9):824-8.
mouse iPSCs
10 M
Differentiation of mouse iPSCs into neurospheres
Bioeng Transl Med. 2022 Sep 10;8(5):e10406.
femoral head chondrocytes
10 μM
3 days
To investigate the effect of retinoic acid on aggrecan degradation in chondrocytes, results showed reduced aggrecan degradation products in Ndst1+/− samples upon RA treatment.
Osteoarthritis Cartilage. 2020 Jul;28(7):977-987.
Neuro-2a (N2A) cells
2 μM
5 days
Induced differentiation of Hb9GFP mouse stem cells into motor neurons
Nat Commun. 2017 Jul 5;8:16063.
iPSC-derived motoneurons
1 µM
4-10 days
Used to induce differentiation of iPSCs into motoneurons, results showed that retinoic acid at 1 µM successfully induced motoneuron differentiation.
Nat Commun. 2015 Jan 12;6:5999.
Retinoic acid/维A酸 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Balb/c mice
Pristane-induced lupus model
Oral
1 mg/kg
Daily administration from 3 weeks to 3 months of age (tRA pre-treatment) or from 3 to 9 months of age (tRA post-treatment)
Investigate the role of tRA at different stages of lupus, finding that tRA pre-treatment exacerbates glomerulonephritis while post-treatment has immunosuppressive effects
Front Immunol. 2020 Mar 20;11:408
Mouse
Embryonic stem cells
Culture medium
5 µM
7 days
Induced differentiation of embryonic stem cells
Sci Adv. 2021 Oct 29;7(44):eabk2775
Mice
Osteoporosis model
Gavage
70 mg/kg
Once daily for 2 weeks
Establish an osteoporosis model, results indicated the model was successfully established
Polymers (Basel). 2018 Feb 14;10(2):185
Mice
Osteoporosis model
Gavage
70 mg/kg
Once daily for 2 weeks
To induce an osteoporosis model in mice by administering retinoic acid, the results showed that serum calcium and ALP levels in the model group were significantly increased, indicating the successful establishment of the osteoporosis model.
Polymers (Basel). 2018 Feb 14;10(2):185
ICR mice
UVB-induced skin damage model
Oral and topical application
2 mg/kg
Once daily for one week
Retinoic acid as the positive control improved UVB-induced skin damage but did not significantly reduce oxidative stress and inflammation, and caused liver damage.
Activity at human RARgamma ligand binding domain expressed in COS7 cells co-transfected with Gal4-DBD assessed as transcriptional activation after 16 hrs by Gal4 response element-driven luciferase reporter gene assay, EC50=0.0002 μM
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