At least 16 h of continuous PY-60 exposure was required for maximal induction of YAP transcriptional response.
Proc Natl Acad Sci U S A. 2023 Jul 11;120(28):e2305085120.
primary adult human epidermal keratinocytes (HEKa)
primary adult human epidermal keratinocytes (HEKa)
24 h
PY-60 induced a robust YAP-driven transcriptional program, reducing terminal differentiation and apoptosis of keratinocytes and increasing proliferative capacity.
Proc Natl Acad Sci U S A. 2023 Jul 11;120(28):e2305085120.
HK-2 cells
HK-2 cells
PY-60 increased the expression of Nrf2, while YAP1 knockdown counteracted the effect of TMP on autophagic flux.
Int J Biol Sci. 2025 Jan 13;21(3):1158-1173.
Mouse primary hepatocytes
Mouse primary hepatocytes
PY-60 significantly inhibited H/R-induced apoptosis in RAMP1-KO primary hepatocytes by suppressing YAP phosphorylation.
J Clin Transl Hepatol. 2024 Apr 28;12(4):357-370.
NCI-H358 cells
NCI-H358 cells
To examine the effect of PY-60 on the Hippo pathway, results showed PY-60 increased YAP protein levels and inhibited its phosphorylation
Cell Div. 2025 Mar 11;20(1):7.
A-549 cells
A-549 cells
To examine the effect of PY-60 on the Hippo pathway, results showed PY-60 increased YAP protein levels and inhibited its phosphorylation
Cell Div. 2025 Mar 11;20(1):7.
PY-60 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Yucatan mini pig
Partial and full thickness wound models
Topical application
0.15 wt% gel
Every other day for 8 days
PY-60 significantly accelerated the closure of partial and full thickness wounds, increasing the thickness of both epidermal and dermal layers.
Proc Natl Acad Sci U S A. 2023 Jul 11;120(28):e2305085120.
Mice
70% liver ischemia-reperfusion model in RAMP1-KO mice
Intraperitoneal injection
10 mg/kg
Single dose, lasting 6 hours
PY-60 significantly alleviated liver ischemia-reperfusion injury by inhibiting YAP phosphorylation and reducing hepatocyte apoptosis.
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