There will be a HazMat fee per item when shipping a dangerous goods. The HazMat fee will be charged to your UPS/DHL/FedEx collect account or added to the invoice unless the package is shipped via Ground service. Ship by air in Excepted Quantity (each bottle), which is up to 1g/1mL for class 6.1 packing group I or II, and up to 25g/25ml for all other HazMat items.
Type
HazMat fee for 500 gram (Estimated)
Excepted Quantity
USD 0.00
Limited Quantity
USD 15-60
Inaccessible (Haz class 6.1), Domestic
USD 80+
Inaccessible (Haz class 6.1), International
USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic
USD 100+
Accessible (Haz class 3, 4, 5 or 8), International
Methotrexate (MTX) is used as an anchor disease-modifying anti-rheumatic drugs (DMARDs) in treating rheumatoid arthritis (RA) because of its potent efficacy and tolerability[3]. Methotrexate is a well-tolerated and effective treatment for allergic contact dermatitis, and shows comparable efficacy to immunomodulatory agents such as cyclosporine and azathioprine, with robust efficacy despite persistent allergen exposure in patients with allergic contact dermatitis[4]. The intrathecal administration of methotrexate can provide therapeutic concentrations in the cerebrospinal fluid (CSF) without the use of high-dose intravenous methotrexate[5]. Formulation consisting prefilled syringe with high concentration of MTX and pre-attached needleis especially suitable for methotrexate subcutaneousself-administration[6]. The dose and duration of MTX therapy for EP (Ectopic pregnancy) is much lower than that used in oncology cases, thus reducing side effects and increasing safety[7].
Methotrexate/甲氨蝶呤 细胞实验
Cell Line
Concentration
Treated Time
Description
References
Ramos cells
20 μM
72 hours
To investigate the effect of FTCD depletion on methotrexate sensitivity, results showed that FTCD depletion significantly reduced the sensitivity of Ramos cells to methotrexate
Nature. 2018 Jul;559(7715):632-636
HEL cells
5 μM
3 days
To investigate the effect of FTCD depletion on methotrexate sensitivity, results showed that FTCD depletion significantly reduced the sensitivity of HEL cells to methotrexate
Nature. 2018 Jul;559(7715):632-636
BHK-CD16a
10 μM
Used for culturing BHK-CD16a cells to study ADCC activity of CD19 antibodies
Front Immunol. 2022 Aug 31;13:957874.
BT-474 cells
0.1 µM
72 h
MTX treatment increases the intracellular concentration of the metabolite AICAR, resulting in AMPK activation. Methotrexate-induced AMPK activation leads to decreased one-carbon metabolism gene expression and cellular proliferation as well as increased global bioenergetic capacity.
Sci Rep. 2020 May 12;10(1):7838.
mouse embryonic fibroblasts (MEFs)
0.1 µM
72 h
MTX caused a strong increase (~30 fold) in endogenous AICAR levels in breast cancer cells and mouse embryonic fibroblasts (MEFs)
Sci Rep. 2020 May 12;10(1):7838.
A549 cells
1 μM
To investigate the effects of MTX on Th17 cell differentiation, results showed that MTX-treated A549 cells co-cultured with PBMCs significantly increased the frequency of Th17 cells.
EBioMedicine. 2024 Oct;108:105339.
Molt4 cells
90 nM
To evaluate the synergy between methotrexate and SHIN2, results showed that methotrexate increased sensitivity to SHIN2
Leukemia. 2021 Feb;35(2):377-388.
Methotrexate/甲氨蝶呤 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
C57BL/6 nude mice
Acute lymphoblastic leukemia mouse model
Intraperitoneal injection
80 mg/kg ginger extract and 5 mg/kg MTX
Five times a week for ginger extract and once a week for MTX, lasting two months
To evaluate the anti-leukemic activity of ginger extract alone or in combination with MTX on ALL mouse models, results showed that combined treatment significantly increased survival rate and reduced damages in brain and liver tissues.
J Cell Mol Med. 2021 May 3;25(13):6148–60
DBA/1J mice
Collagen-induced arthritis (CIA) mouse model
Intraperitoneal injection
23 mg/kg
Thrice a week for 38 days
To evaluate the effect of MTX on arthritis in CIA mice, the results showed that MTX significantly alleviated arthritis symptoms in CIA mice.
Clin Transl Med. 2022 Dec;12(12):e1148.
DBA/1J female mice
Bovine collagen-induced arthritis (CIA) model
Intragastric administration
0.92 mg/kg
Twice a week for 30 days
To evaluate the therapeutic effect of Methotrexate on arthritis in CIA mice, the results showed that Methotrexate was effective in controlling inflammation but had no obvious benefits in regulating weight loss and mechanical allodynia
Front Pharmacol. 2021 May 28;12:693777
DBA/1 mice
Collagen-induced arthritis (CIA) model
Oral gavage
2 mg/kg
Every 3 days for 20 days
To evaluate the therapeutic effect of MTX on CIA model mice, the results showed that MTX significantly alleviated arthritis symptoms and footpad swelling.
Front Immunol. 2018 Nov 27;9:2762
SKG mice
RA-ILD model
Intraperitoneal injection
7.5 mg/kg
Twice weekly for 12 weeks
To investigate the effects of MTX on pulmonary inflammation in the RA-ILD mouse model, results showed that MTX exacerbated pulmonary inflammation, increased immune cell infiltration, and fibrosis.
EBioMedicine. 2024 Oct;108:105339.
Mice
NOTCH1-driven mouse primary T-ALL model
Intraperitoneal injection
10 mg/kg
Administered every 5 days for 4 weeks
To evaluate the synergy between methotrexate and SHIN2, results showed that the combination significantly extended mouse survival
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