There will be a HazMat fee per item when shipping a dangerous goods. The HazMat fee will be charged to your UPS/DHL/FedEx collect account or added to the invoice unless the package is shipped via Ground service. Ship by air in Excepted Quantity (each bottle), which is up to 1g/1mL for class 6.1 packing group I or II, and up to 25g/25ml for all other HazMat items.
Type
HazMat fee for 500 gram (Estimated)
Excepted Quantity
USD 0.00
Limited Quantity
USD 15-60
Inaccessible (Haz class 6.1), Domestic
USD 80+
Inaccessible (Haz class 6.1), International
USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic
USD 100+
Accessible (Haz class 3, 4, 5 or 8), International
Dynamin is a large GTPase enzyme that severs membrane‐bound clathrin‐coated vesicles. Dyngo-4a inhibits both dynamin I and II with IC50s for sheep brain dynamin I of 380 ± 0.05 nM (n = 5) and for recombinant rat dynamin II of 2.3 ± 0.2 μM (n = 3). It inhibits dynamin‐dependent endocytosis of transferrin in multiple cell types with IC50 value of 5.7 μM. Inhibition of non‐neuronal clathrin‐mediated endocytosis (CME) was observed with as little as 5‐min preincubation with 10 μM Dyngo-4a in U2OS cells. 70% of normal non‐neuronal CME levels was observed at ten minutes after the removal of 10 μM 4a indicating its endocytic block activity was rapid and reversible [3]. The ability of Dyngo-4a to prevent the muscle paralysis induced by purified BoNT/A was tested in a rat hemidiaphragm twitch model. Both the decline in contraction and the time to reach 50% of decline were significantly increased in the Dyngo-4a-treated muscles compared with the BoNT/A control group demonstrating that Dyngo-4a provides significant protection against BoNT/A-induced muscle paralysis. CD-1 mice were given an intraperitoneal injection of Dyngo-4a (1 mg) or vehicle followed by a booster 4.5–8 h later. Mice injected with Dyngo-4a took significantly longer to exhibit clear signs of botulism, 860 ± 65 min compared with 656 ± 55 min indicating that Dyngo-4a pretreatment provides significant protection against botulism [4].
Hydroxy-Dynasore 细胞实验
Cell Line
Concentration
Treated Time
Description
References
Neuro2A cells
30 µM
30 minutes
Inhibited OGD-induced KOR internalization
Anesthesiology. 2017 May;126(5):842-854.
HEK293 cells
30 µM
15 min
To assess the effects of Dyngo on β2-AR internalization and cAMP accumulation.
Nat Chem Biol. 2014 Dec;10(12):1061-5.
Rat Mesenchymal Stem Cells
20 µM
15 minutes
To investigate the effect of Dyngo-4a on the internalization of S100B-Alexa488, results showed that Dyngo-4a significantly reduced the number and mean fluorescence intensity of S100B-Alexa488-positive vesicles.
Front Cell Dev Biol. 2021 Jul 26;9:674995.
Xenopus laevis oocytes
10 µM
20 hours
Dyngo-4a significantly increased TMEM175 currents, indicating that it enhanced the expression of TMEM175 on the plasma membrane by inhibiting dynamin.
Int J Mol Sci. 2021 Sep 29;22(19):10515.
HEK293T cells
80 µM
20 hours
Dynasore increased the expression of TMEM175 on the plasma membrane, validating its functional expression on the cell surface.
Int J Mol Sci. 2021 Sep 29;22(19):10515.
Human foreskin keratinocytes (HFK)
10 µM
24 hours
Dyngo-4a-treated shIRF6 keratinocytes showed increased levels of E-cadherin at the membrane to the levels of the wild-type cells.
J Invest Dermatol. 2022 Feb;142(2):314-322.
HUVECs
30 µM
30 minutes
To evaluate the effect of dyngo 4a on VEGFR2 internalization and signaling, results showed that dyngo 4a significantly inhibited VEGFR2 phosphorylation.
Cells. 2021 Apr 23;10(5):997.
Inner hair cells (IHCs)
30 µM
Pharmacological intervention with dynamin-mediated endocytosis; no significant effect observed on cumulative exocytic ΔCm.
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