货号:A2924130
同义名:
重组人pro-Beta NGF蛋白(Tag free, E.coli)
/ Recombinant Human pro-Beta NGF (E.coli); Beta-Nerve Growth Factor
Recombinant Human pro-Beta NGF (E.coli)是人源蛋白,在大肠杆菌中表达,为无标签形式。该产品为神经生长因子前体形式(proNGF),与成熟形式均具有半胱氨酸结结构,但与成熟神经生长因子作用相反,主要通过p75神经营养因子受体和sortilin受体复合物发挥作用,促进神经元凋亡,参与年龄及疾病相关的神经退行性病变。


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| 描述 | The precursor form of the nerve growth factor (proNGF) like its mature form is characterized by the cystin knot motif consisting of three cystine bridges, whereas proneurotrophins and mature neurotrophins elicit opposite biological effects. ProNGF functions preferentially via the complex of pan-neurotrophin receptor p75 (p75NTR) and vps10p domain-containing receptor sortilin inducing neuronal apoptosis and contributing to age- and disease-related neurodegeneration. |
| 蛋白种属 | Human |
| 表达系统 | E.coli |
| 标签 | Tag free |
| 蛋白表达区间 | Glu19-Ala241 |
| 蛋白编号 | P01138 |
| 内毒素 | < 1 EU/µg as determined by LAL test. |
| 剂型 | 干粉 |
| 剂型描述 | Lyophilized from a 0.2 μm filtered solution of 20mM Histidine-HCl,5%Sucrose,0.02%Tween80,pH5.0. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存,建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释,如果是做无血清培养或者动物体内实验时,细胞因子中不能含有BSA、FBS 和 HAS,可以使用海藻糖作为载体,然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子,并避免反复冻融;稀释分装浓度不低于 10μg/mL,分装体积不低于10μL。 分装后的蛋白在≤-20°C 下可稳定保存 3 个月。 |
| CAS号 | N/A |
| 分子量 | 25 KDa |
| SMILES Code | NONE |
| 别名 | 重组人pro-Beta NGF蛋白(Tag free, E.coli) ;Recombinant Human pro-Beta NGF (E.coli); Beta-Nerve Growth Factor; Beta-NGF; NGF; NGFB; β-NGF; pro-Beta NGF |
| 运输 | 蓝冰 |
| 存储条件 |
In solvent -20°C: 3-6个月 -80°C: 12个月 Pure form Sealed in dry, store in freezer, under -20°C |
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