货号:A2924064
同义名:
重组人PDGF-AA蛋白(N-6His, E.coli)
/ Recombinant Human PDGF-AA (N-6His, E.coli); PDGFAA
Recombinant Human PDGF-AA (N-6His, E.coli) 是人源血小板衍生生长因子亚基A,在大肠杆菌中表达,带有N端6×His标签。该蛋白属于PDGF/VEGF生长因子家族,是一种分泌蛋白,可促进平滑肌细胞、结缔组织细胞、骨与软骨细胞及多种血细胞的增殖、迁移与存活,参与胚胎发育、伤口愈合及器官形成,通过与PDGFB形成异源二聚体调节信号传导。


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| 描述 | Platelet-derived growth factor subunit A (PDGFA), belongs to the PDGF/VEGF growth factor family. PDGFA is a secreted protein, stored in platelet alpha-granules and released by platelets upon wounding. PDGFA is potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. It plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. PDGFA is required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis, normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. It plays an important role in wound healing; Signaling is modulated by the formation of heterodimers with PDGFB. |
| 蛋白种属 | Human |
| 表达系统 | E.coli |
| 标签 | N-6His |
| 蛋白表达区间 | Ser87-Thr211 |
| 蛋白编号 | P04085 |
| 内毒素 | < 1 EU/µg as determined by LAL test. |
| 剂型 | 干粉 |
| 剂型描述 | Lyophilized from a 0.2 μm filtered solution of 4mM HCl. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in 4mM HCl. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存,建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释,如果是做无血清培养或者动物体内实验时,细胞因子中不能含有BSA、FBS 和 HAS,可以使用海藻糖作为载体,然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子,并避免反复冻融;稀释分装浓度不低于 10μg/mL,分装体积不低于10μL。 分装后的蛋白在≤-20°C 下可稳定保存 3 个月。 |
| CAS号 | N/A |
| 分子量 | 15.9 KDa |
| SMILES Code | NONE |
| 别名 | 重组人PDGF-AA蛋白(N-6His, E.coli) ;Recombinant Human PDGF-AA (N-6His, E.coli); PDGFAA; PDGF-AA |
| 运输 | 蓝冰 |
| 存储条件 |
In solvent -20°C: 3-6个月 -80°C: 12个月 Pure form Sealed in dry, store in freezer, under -20°C |
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