产品名称：Recombinant Human Noggin理论分子量：23 KDa蛋白种属：Human表达系统：Human Cells蛋白表达区间：Gln28-Cys232标签：Tag free剂型：干粉纯度：≥95% as determined by reducing SDS-PAGE.储存条件：冻干蛋白应储存在≤-20°C，收到后可稳定保存一年。
溶解后的蛋白溶液可以在2-8°C下保存2-7天。如要长期保存，建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释，如果是做无血清培养或者动物体内实验时，细胞因子中不能含有BSA、FBS 和 HAS，可以使用海藻糖作为载体，然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子，并避免反复冻融；稀释分装浓度不低于 10μg/mL，分装体积不低于10μL。
分装后的蛋白在≤-20°C 下可稳定保存 3 个月。产品背景：Noggin is a secreted homodimeric glycoprotein that is an antagonist of bone morphogenetic proteins (BMPs). Mature Human Noggin contains an N-terminal acidic region, a central basic heparin-binding segment and a C-terminal cysteine-knot structure. Noggin is very highly conserved among vertebrates, such that mature human Noggin shares 99%, 99%, 98%, 97% and 89% aa sequence identity with mouse, rat bovine, equine and chicken Noggin, respectively. Secreted Noggin probably remains close to the cell surface due to its binding of heparin-containing proteoglycans. Noggin binds some BMPs such as BMP4 with high affinity and others such as BMP7 with lower affinity. It antagonizes BMP bioactivities by blocking epitopes on BMPs that are needed for binding to both type I and type II receptors. Noggin is expressed in defined areas of the adult central nervous system and peripheral tissues such as lung, skeletal muscle and skin. During culture of human embryonic stem cells (hESC) or neural stem cells under certain conditions, addition of Noggin to antagonize BMP activity may allow stem cells to proliferate while maintaining their undifferentiated state, or alternatively, to differentiate into dopaminergic neurons.
Human Noggin/人Noggin蛋白(HEK293, tag free) 技术信息
CAS号
N/A
分子量
23 KDa
SMILES Code
NONE
别名
重组人Noggin蛋白(HEK293, tag free)
;Recombinant Human Noggin
运输
蓝冰
存储条件
In solvent-20°C:3-6个月-80°C:12个月
Pure formSealed in dry, store in freezer, under -20°C
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Tags:
Human Noggin/人Noggin蛋白(HEK293, tag free)
|Human Noggin/人Noggin蛋白(HEK293, tag free)
| 重组人Noggin蛋白(HEK293, tag free)
| Recombinant Human Noggin
| N/A
| Other Cytokines and Growth Factors
| 干细胞诱导
| Human Noggin/人Noggin蛋白(HEK293, tag free) 纯度/质量文件
| Human Noggin/人Noggin蛋白(HEK293, tag free) 生物活性
| Human Noggin/人Noggin蛋白(HEK293, tag free) 技术信息
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