Recombinant Human MAG (C-6His, HEK293)是人源蛋白，在HEK293细胞中表达，带有C端6His标签。该蛋白即人髓鞘相关糖蛋白，属于SIGLEC家族，含4个Ig样C2型结构域和1个Ig样V型结构域，作为粘附分子参与神经发育及髓鞘形成，介导神经元与髓鞘细胞间依赖唾液酸的细胞间相互作用，尤其偏好结合α-2,3-连接的唾液酸，其可溶性形式在多种组织中存在，可能影响中枢神经损伤后的再生。
Human MAG (C-6His, HEK293)/人MAG蛋白(C-6His, HEK293) 化学结构 CAS号：N/A
Human MAG (C-6His, HEK293)/人MAG蛋白(C-6His, HEK293) 3D分子结构 CAS号：N/A
Human MAG (C-6His, HEK293)/人MAG蛋白(C-6His, HEK293) 质控信息
Human MAG (C-6His, HEK293)/人MAG蛋白(C-6His, HEK293) 生物活性
描述
Human Myelin-Associated Glycoprotein,also known as MAG, Siglec-4,is a cell membrane glycoprotein that is a member of the SIGLEC family of proteins.MAG contains 4 Ig-like C2-type domains and 1 Ig-like V-type domain.MAG functions as an adhesion molecule during neural development. MAG is believed to be involved in myelination during nerve regeneration. it is a adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells and Preferentially binds to alpha-2,3-linked sialic acid. Soluble MAG, which is released from myelin in large quantities, has been identified in normal human tissues and in tissues from patients with neurological disorders. It is believed that this soluble MAG might contribute to the lack of CNS neuron regeneration after injury.
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
复溶
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存，建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释，如果是做无血清培养或者动物体内实验时，细胞因子中不能含有BSA、FBS 和 HAS，可以使用海藻糖作为载体，然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子，并避免反复冻融；稀释分装浓度不低于 10μg/mL，分装体积不低于10μL。
分装后的蛋白在≤-20°C 下可稳定保存 3 个月。
Human MAG (C-6His, HEK293)/人MAG蛋白(C-6His, HEK293) 技术信息
搜索
