Recombinant Human M-CSF (E.coli) 是人源巨噬细胞集落刺激因子，在大肠杆菌中表达，不含标签。该蛋白在造血过程中调控粒细胞与单核-巨噬细胞的生成、分化及功能，促进促炎趋化因子释放，参与先天免疫与炎症反应，调节破骨细胞增殖与分化及骨吸收，对正常骨发育、生殖功能至关重要，并影响细胞骨架重组、膜皱褶形成、细胞黏附与迁移，以及脂蛋白清除。
Human M-CSF (E.coli)/人M-CSF蛋白(Tag free, E.coli) 化学结构 CAS号：N/A
Human M-CSF (E.coli)/人M-CSF蛋白(Tag free, E.coli) 3D分子结构 CAS号：N/A
Human M-CSF (E.coli)/人M-CSF蛋白(Tag free, E.coli) 质控信息
Human M-CSF (E.coli)/人M-CSF蛋白(Tag free, E.coli) 生物活性
描述
Macrophage Colony-Stimulating Factors (m-csf) are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and themonocytes-macrophages. CSF-1 promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. It also plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. CSF-1 is required for normal male and female fertility and promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. it also plays a role in lipoprotein clearance.
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 3% Trehalase, 2% Mannitol, 0.02% Tween 80, pH7.4.
复溶
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存，建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释，如果是做无血清培养或者动物体内实验时，细胞因子中不能含有BSA、FBS 和 HAS，可以使用海藻糖作为载体，然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子，并避免反复冻融；稀释分装浓度不低于 10μg/mL，分装体积不低于10μL。
分装后的蛋白在≤-20°C 下可稳定保存 3 个月。
Human M-CSF (E.coli)/人M-CSF蛋白(Tag free, E.coli) 技术信息
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