Leukemia Inhibitory Factor (LIF) is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo. Human and murine mature LIF exhibit a 78% sequence identity at the amino acid level. Human LIF is equally active on human and mouse cells. Murine LIF is approximately 1000 fold less active on human cells than human LIF.
Lyophilized from a 0.2 μm filtered solution of 20mM PB,2% Sucrose,4% Mannitol,0.02% Tween 80,pH7.4.
复溶
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存，建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释，如果是做无血清培养或者动物体内实验时，细胞因子中不能含有BSA、FBS 和 HAS，可以使用海藻糖作为载体，然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子，并避免反复冻融；稀释分装浓度不低于 10μg/mL，分装体积不低于10μL。
分装后的蛋白在≤-20°C 下可稳定保存 3 个月。
Human LIF/人LIF蛋白(E.coli, tag free) 技术信息
CAS号
N/A
分子量
19.7 KDa
SMILES Code
NONE
别名
重组人LIF蛋白(E.coli, tag free)
;Recombinant Human LIF; Leukemia Inhibitory Factor; LIF; Differentiation-Stimulating Factor; D Factor; Melanoma-Derived LPL Inhibitor; MLPLI; Emfilermin; LIF; HILDA
运输
蓝冰
存储条件
In solvent-20°C:3-6个月-80°C:12个月
Pure formSealed in dry, store in freezer, under -20°C
最近浏览的产品
Tags:
Human LIF/人LIF蛋白(E.coli, tag free)
|Human LIF/人LIF蛋白(E.coli, tag free)
| 重组人LIF蛋白(E.coli, tag free)
| Recombinant Human LIF
| Leukemia Inhibitory Factor
| LIF
| Differentiation-Stimulating Factor
| D Factor
| Melanoma-Derived LPL Inhibitor
| MLPLI
| Emfilermin
| LIF
| HILDA
| N/A
| Neurotrophic Factors
| 干细胞诱导
| Human LIF/人LIF蛋白(E.coli, tag free) 纯度/质量文件
| Human LIF/人LIF蛋白(E.coli, tag free) 生物活性
| Human LIF/人LIF蛋白(E.coli, tag free) 技术信息
搜索
