货号:A2926476
同义名:
重组人IGHE蛋白(104-427, C-6His, HEK293)
/ Recombinant Human IGHE (104-427, C-6His, HEK293); Immunoglobulin heavy constant epsilon
Recombinant Human IGHE (104-427, C-6His, HEK293) 是人源免疫球蛋白重链恒定区ε(IgE)蛋白,在人源细胞中表达,带有C端6×His标签。该蛋白即IgE,是引发速发型过敏反应的关键抗体,由B淋巴细胞经类别转换产生,其膜结合形式的恒定区是B细胞受体复合物的组成部分,可介导抗原交联触发的BCR信号,促进IgE转换B细胞存活与浆细胞分化,调控初始及记忆性IgE应答。


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| 描述 | Immunoglobulin heavy constant epsilon is knowed as Immunoglobulin (Ig) E, which causes the immediate allergic reaction, is produced by B lymphocytes through the class switching. Constant region of membrane-bound IgE (long mIgE), part of the B cell receptor complex (BCR). Upon antigen cross-linking triggers quick BCR signaling, ensuring survival of IgE-switched B cells and differentiation into plasma cells, thus regulating both primary and memory IgE responses. |
| 蛋白种属 | Human |
| 表达系统 | Human Cells |
| 标签 | C-6His |
| 蛋白表达区间 | Cys104-Lys427 |
| 蛋白编号 | AAB59395.1 |
| 内毒素 | < 1 EU/µg as determined by LAL test. |
| 剂型 | 干粉 |
| 剂型描述 | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存,建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释,如果是做无血清培养或者动物体内实验时,细胞因子中不能含有BSA、FBS 和 HAS,可以使用海藻糖作为载体,然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子,并避免反复冻融;稀释分装浓度不低于 10μg/mL,分装体积不低于10μL。 分装后的蛋白在≤-20°C 下可稳定保存 1 个月。 |
| CAS号 | N/A |
| 分子量 | 37.1 KDa |
| SMILES Code | NONE |
| 别名 | 重组人IGHE蛋白(104-427, C-6His, HEK293) ;Recombinant Human IGHE (104-427, C-6His, HEK293); Immunoglobulin heavy constant epsilon; Ig epsilon chain C region; Ig epsilon chain C region ND; IGHE |
| 运输 | 蓝冰 |
| 存储条件 |
In solvent -20°C: 3-6个月 -80°C: 12个月 Pure form Sealed in dry, store in freezer, under -20°C |
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