货号:A2926014
同义名:
重组人GSH-S蛋白(C-6His, E.coli)
/ Recombinant Human GSH-S (C-6His, E.coli); Glutathione Synthetase
Recombinant Human GSH-S (C-6His, E.coli) 是人源谷胱甘肽合成酶,在大肠杆菌中表达,带有C端6×His标签。该蛋白属于真核生物GSH合成酶家族,是谷胱甘肽生物合成途径中的第二种酶,催化γ-谷氨酰半胱氨酸与甘氨酸缩合形成谷胱甘肽,对解毒、抗氧化及膜转运等生理功能至关重要,以同源二聚体形式发挥ATP依赖的催化作用。


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| 描述 | Glutathione Synthetase belongs to the eukaryotic GSH synthase family. Glutathione Synthetase is the second enzyme in the glutathione biosynthesis pathway. It catalyses the condensation of gamma-glutamylcysteine and glycine to form glutathione. Glutathione play an important role in a variety of biological functions, including detoxification of xenobiotics, protection of cells from oxidative damage by free radicals, and membrane transport. The protein functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in Glutathione Synthetase can also cause the glutathione synthetase deficiency of erythrocytes, which is a mild form causing hemolytic anemia. |
| 蛋白种属 | Human |
| 表达系统 | E.coli |
| 标签 | C-6His |
| 蛋白表达区间 | Ala2-Val474 |
| 蛋白编号 | P48637 |
| 内毒素 | < 1 EU/µg as determined by LAL test. |
| 剂型 | 液体 |
| 剂型描述 | Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 200mM NaCl, pH 7.5. |
| 复溶 | 如要长期保存,建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释,如果是做无血清培养或者动物体内实验时,细胞因子中不能含有BSA、FBS 和 HAS,可以使用海藻糖作为载体,然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子,并避免反复冻融;稀释分装浓度不低于 10μg/mL,分装体积不低于10μL。分装后的蛋白在≤-20°C 下可稳定保存 3 个月。 |
| CAS号 | N/A |
| 分子量 | 53.45 KDa |
| SMILES Code | NONE |
| 别名 | 重组人GSH-S蛋白(C-6His, E.coli) ;Recombinant Human GSH-S (C-6His, E.coli); Glutathione Synthetase; GSH Synthetase; GSH-S; Glutathione Synthase; GSS |
| 运输 | 蓝冰 |
| 存储条件 |
In solvent -20°C:3-6个月-80°C:12个月 |
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