Growth Differentiation Factor 5(GDF-5, BMP-14) is a member of the BMP family of TGFβ superfamily proteins. Human GDF-5, -6, and -7 are a defined subgroup of the BMP family. GDF-5 is synthesized as a homodimeric precursor protein consisting of a 354 amino acid (aa) Nterminal proregion and a 120 aa C-terminal mature peptide. Mature human GDF-5 shares 99% aa sequence identity with both mature mouse and rat GDF-5. GDF-5 signaling is mediated by formation of a heterodimeric complex consisting of a type 1 (BMPR-IB) and a type II (BMPR-IIor Activin RII) serine/threonine kinase receptor which results in the phosphorylation and activation of cytosolic Smad proteins (Smad1, 5, and 8). GDF-5 is involved in multiple developmental processes including limb generation, cartilage development, joint formation, bone morphogenesis, cell survival, and neuritogenesis. Inhibition of GDF-5 expression or alteration of its signaling can facilitate the development of osteoarthritis.
Lyophilized from a 0.2 μm filtered solution of 4mM HCl.
复溶
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in 4mM HCl.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存，建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释，如果是做无血清培养或者动物体内实验时，细胞因子中不能含有BSA、FBS 和 HAS，可以使用海藻糖作为载体，然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子，并避免反复冻融；稀释分装浓度不低于 10μg/mL，分装体积不低于10μL。
分装后的蛋白在≤-20°C 下可稳定保存 3 个月。
Human GDF-5/人GDF-5蛋白(E.coli, tag free) 技术信息
CAS号
N/A
分子量
13.7 KDa
SMILES Code
NONE
别名
重组人GDF-5蛋白(E.coli, tag free)
;Recombinant Human GDF-5; Growth/differentiation factor 5; GDF-5; Bone morphogenetic protein 14; BMP-14; Cartilage-derived morphogenetic protein 1; CDMP-1; Lipopolysaccharide-associated protein 4; LAP-4; LPS-associated protein 4; Radotermin; CDMP1
运输
蓝冰
存储条件
In solvent-20°C:3-6个月-80°C:12个月
Pure formSealed in dry, store in freezer, under -20°C
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Tags:
Human GDF-5/人GDF-5蛋白(E.coli, tag free)
|Human GDF-5/人GDF-5蛋白(E.coli, tag free)
| 重组人GDF-5蛋白(E.coli, tag free)
| Recombinant Human GDF-5
| Growth/differentiation factor 5
| GDF-5
| Bone morphogenetic protein 14
| BMP-14
| Cartilage-derived morphogenetic protein 1
| CDMP-1
| Lipopolysaccharide-associated protein 4
| LAP-4
| LPS-associated protein 4
| Radotermin
| CDMP1
| N/A
| TGF-beta Superfamily
| 干细胞诱导
| Human GDF-5/人GDF-5蛋白(E.coli, tag free) 纯度/质量文件
| Human GDF-5/人GDF-5蛋白(E.coli, tag free) 生物活性
| Human GDF-5/人GDF-5蛋白(E.coli, tag free) 技术信息
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