Human B7-H3 (2Ig, C-Fc, HEK293)/人B7-H3蛋白(2Ig, C-Fc, HEK293) 质控信息
Human B7-H3 (2Ig, C-Fc, HEK293)/人B7-H3蛋白(2Ig, C-Fc, HEK293) 生物活性
描述
CD276, also known as B7-H3, is a member of the B7 superfamily with signature IgV and IgG regions in extracellular domains. It is also reported that B7-H3 may play an important role in muscle-immune interactions, providing further evidence of the active role of muscle cells in local immunoregulatory processes. B7-H3 is expressed on T-cells, natural killer cells, and antigen presenting cells, as well as some non-immune cells, such as osteoblasts, fibroblasts, fibroblast-like synoviocytes and epithelial cells. High expression of B7-H3 in tumor vasculature also correlates with poor survival in patients, suggesting that it may play a role in tumor cell migration. In humans, the B7-H3 gene has four Ig-like repeats that can be alternatively spliced to yield a protein containing either four Ig-like (4Ig) or two Ig-like (2Ig) domains, whereas the mouse gene only contains two Ig-like repeats and thus, only one form (2Ig) is generated. Systemic genomic analysis suggested that the 4Ig form, with two copies of IgV-IgC domain, was generated from tandem exon duplication during evolution of the immune system.
Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
复溶
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存，建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释，如果是做无血清培养或者动物体内实验时，细胞因子中不能含有BSA、FBS 和 HAS，可以使用海藻糖作为载体，然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子，并避免反复冻融；稀释分装浓度不低于 10μg/mL，分装体积不低于10μL。
分装后的蛋白在≤-20°C 下可稳定保存 1 个月。
Human B7-H3 (2Ig, C-Fc, HEK293)/人B7-H3蛋白(2Ig, C-Fc, HEK293) 技术信息
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