There will be a HazMat fee per item when shipping a dangerous goods. The HazMat fee will be charged to your UPS/DHL/FedEx collect account or added to the invoice unless the package is shipped via Ground service. Ship by air in Excepted Quantity (each bottle), which is up to 1g/1mL for class 6.1 packing group I or II, and up to 25g/25ml for all other HazMat items.
Type
HazMat fee for 500 gram (Estimated)
Excepted Quantity
USD 0.00
Limited Quantity
USD 15-60
Inaccessible (Haz class 6.1), Domestic
USD 80+
Inaccessible (Haz class 6.1), International
USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic
USD 100+
Accessible (Haz class 3, 4, 5 or 8), International
HJC0350 is a selective EPAC2 antagonist with IC50 value of 0.3μM for competing with 8-NBD-cAMP binding of EPAC2[3]. HJC0350 at 0.3μM represented inhibitory effects of the insulinotropic function of AWRK6 in the mouse pancreatic β cell line Min6 cells[4].
作用机制
HJC0350 inhibited EPAC2 in a competitive manner.[3]
HJC0350 细胞实验
Cell Line
Concentration
Treated Time
Description
References
Min6 cells
0.3 μM
30 minutes
inhibitory effects of the insulinotropic function of AWRK6
Int J Mol Sci. 2018 Oct 7;19(10):3053
HEK293/AKAR3 cells
10 µM
To test the inhibitory effect of HJC0350 on PKA, results showed that HJC0350 did not affect the activation of PKA by 8-Br-cAMP-AM
J Med Chem. 2013 Feb 14;56(3):952-62
HEK293/EPAC2-FL cells
10 µM
To test the inhibitory effect of HJC0350 on EPAC2, results showed that HJC0350 fully blocked the 007-AM induced FRET changes
J Med Chem. 2013 Feb 14;56(3):952-62
RAW 264.7 cells
0.3 µmol/L
24 hours
Evaluate the effect of HJC-0350 on inflammatory response in RAW 264.7 cells. Results showed that HJC-0350 enhanced phosphorylation of NF-κB p65 and p38 MAPK.
J Cell Mol Med. 2022 Jan;26(1):216-227
Caco-2 cells
0.3 µmol/L
24 hours
Evaluate the effect of HJC-0350 on LPS-induced damage in Caco-2 cells. Results showed that HJC-0350 did not ameliorate LPS-induced monolayer damage.
J Cell Mol Med. 2022 Jan;26(1):216-227
HJC0350 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Il-10−/− mice
Spontaneous colitis model
Intraperitoneal injection
0.5 mg/kg
Every 2 days for 4 weeks
Evaluate the effect of HJC-0350 on spontaneous colitis. Results showed that HJC-0350 exacerbated colitis symptoms, increased inflammatory factor expression, and intestinal permeability.
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