货号:A2926177
同义名:
重组大肠杆菌Trp synthase蛋白(N-6His, E.coli)
/ Recombinant E.coli Trp synthase (N-6His, E.coli); Tryptophan synthetase
Recombinant E.coli Trp synthase (N-6His)是大肠杆菌来源的Tryptophan synthase重组蛋白,在大肠杆菌表达系统中表达,带有N端6×His标签。该蛋白是由α和β两个亚基组成的多功能酶复合物,催化L-色氨酸合成的最后两步反应,其中α亚基水解3-吲哚-D-甘油-3′-磷酸,β亚基在吡哆醛5′-磷酸依赖下催化后续反应。该酶广泛存在于原核生物及部分真核生物中,但人类不表达,具有潜在药物靶点价值。


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| 描述 | Tryptophan synthase is a multienzyme α2β2 complex composed of two protein subunit. Tryptophan synthase catalyzes the last two steps in the synthesis of L-tryptophan (L-Trp). The α-subunit catalyzes cleavage of 3-indole-d-glycerol 3′-phosphate (IGP) to give indole and D-glyceraldehyde 3′-phosphate (G3P). Indole is then transferred through a 25-Å hydrophobic tunnel to the β-subunit. The β2 subunit contains pyridoxal 5'-phosphate and catalyzes several pyridoxal 5'-phosphate-dependent reactions, including/3-elimination reactions 6 and a thiol-dependent transamination reaction. This enzyme is commonly found in Eubacteria, Archaebacteria, Protista, Fungi, and Plantae, but is absent from Animalia. As humans do not have tryptophan synthase, this enzyme has been explored as a potential drug target. |
| 蛋白种属 | E.coli |
| 表达系统 | E.coli |
| 标签 | N-6His |
| 蛋白表达区间 | Met1-Ser268&Thr2-Ile397 |
| 蛋白编号 | P0A877, P0A879 |
| 内毒素 | < 1 EU/µg as determined by LAL test. |
| 剂型 | 液体 |
| 剂型描述 | Supplied as a 0.2 μm filtered solution of PBS, pH 7.4. |
| 复溶 | 如要长期保存,建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释,如果是做无血清培养或者动物体内实验时,细胞因子中不能含有BSA、FBS 和 HAS,可以使用海藻糖作为载体,然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子,并避免反复冻融;稀释分装浓度不低于 10μg/mL,分装体积不低于10μL。分装后的蛋白在≤-20°C 下可稳定保存 3 个月。 |
| CAS号 | N/A |
| 分子量 | 28.7&43.8 KDa |
| SMILES Code | NONE |
| 别名 | 重组大肠杆菌Trp synthase蛋白(N-6His, E.coli) ;Recombinant E.coli Trp synthase (N-6His, E.coli); Tryptophan synthetase; Tryptophan synthase |
| 运输 | 蓝冰 |
| 存储条件 |
In solvent -20°C:3-6个月-80°C:12个月 |
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