Castanospermine inhibits all forms of α- and β-glucosidases, especially glucosidase l (required for glucoprotein processing by transfer of mannose and glucose from asparagine-linked lipids).
Castanospermine/亚胺糖栗树精胺 细胞实验
Cell Line
Concentration
Treated Time
Description
References
HepG2 cells
0.5 mM
Prolonged the association of transferrin with chaperones (calnexin and calreticulin), prevented completion of folding, and promoted aggregate formation
EMBO J. 1997 Sep 1;16(17):5420-32
Peripheral blood mononuclear cells (PBMCs)
169-339 μM
14 days
Inhibited HIV-1 and HIV-2 replication, but the effects were less persistent compared to H9 cells
Evaluate the inhibitory effect of Castanospermine on DEN-2 in Huh-7 cells, showing an IC50 of 85.7 μM.
J Virol. 2005 Jul;79(14):8698-706
BHK-21 cells
1 μM (IC50)
72 h
Evaluate the inhibitory effect of Castanospermine on DEN-2, showing an IC50 of 1 μM in BHK-21 cells.
J Virol. 2005 Jul;79(14):8698-706
2B4 T hybridoma cells
100 μg/ml
3 h
Inhibition of glucosidase activity severely impaired the assembly of clonotypic TCRα with TCRβ, leading to accelerated degradation of TCRα proteins and failure to form αβ dimers.
EMBO J. 1994 Aug 15;13(16):3678-86
BW5147 T cells
100 μg/ml
3 h
Inhibition of glucosidase activity resulted in markedly accelerated degradation of nascent TCRα proteins with a half-life of ~20 min, while TCRβ protein stability was unaffected.
EMBO J. 1994 Aug 15;13(16):3678-86
canine pancreas microsomes
1 mM
1 h
To study the effect of CST on the binding of HA to CNX and CRT, results showed that CST inhibited the binding of HA to CNX and CRT.
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