Cardamonin, a chalcone extracted from cardamom spice with anti-inflammatory and anti-tumor activities. Cardamonin decreased viability of NPC(Nasopharyngeal carcinoma ) cells in a concentration-dependent manner. The IC50 values were 16.22 μM(CNE-1), 14.34 μM (CNE-2), 16.50 μM (HONE-1), and 68.12 μM (SUNE-1). Moreover, the expression level of the proapoptotic effector protein Bax and Bid was also markedly increased after treatment with cardamonin（15μM） in CNE-2 cells. In vivo, cardamonin (25 mg/kg once every other day from days 13) enhances chemotherapy efficacy.
Cardamonin/豆蔻明 细胞实验
Cell Line
Concentration
Treated Time
Description
References
MDA-MB-231 cells
20 μM
2, 4, 6 h
Cardamonin increased ROS levels in MDA-MB-231 cells
J Exp Clin Cancer Res. 2019 Aug 27;38(1):377.
MDA-MB-231 cells
20 μM
6 h
Cardamonin decreased glucose uptake in MDA-MB-231 cells
J Exp Clin Cancer Res. 2019 Aug 27;38(1):377.
MDA-MB-231 cells
20 μM
3, 6, 12 h
Cardamonin enhanced mitochondrial oxidative phosphorylation in MDA-MB-231 cells
J Exp Clin Cancer Res. 2019 Aug 27;38(1):377.
RAW 264.7 cells
10, 20, 30 µM
24 h
Cardamonin inhibited nitric oxide production in LPS-stimulated RAW 264.7 cells without affecting cell viability.
Biomolecules. 2021 Apr 29;11(5):661.
THP-1 cells
20 µM
24 h
Conditioned medium from cardamonin-pre-treated THP-1 cells did not increase the proliferation of HCT116 cells.
Biomolecules. 2021 Apr 29;11(5):661.
Nf1-silenced murine GBM cells
10 and 20 μM
24 and 48 h
To evaluate the effect of Cardamonin on NF-κB phosphorylation, results showed that Cardamonin treatment did not reduce NF-κB phosphorylation
J Clin Invest. 2023 Nov 15;133(22):e163802.
THP-1 induced macrophages
10 µM and 20 µM
24 h
Cardamonin suppressed M2 polarization of TAMs and downregulated TAM-secreted tumorigenic factors, thereby hindering the pro-tumor function of TAMs on ovarian cancer cells.
Mol Ther Oncolytics. 2022 Jun 25;26:175-188.
SKOV3 and A2780 cells
0, 10, 20, 30, 40, 50 µM
24 h
Cardamonin inhibited the cell viability of SKOV3 and A2780 cells in a concentration-dependent manner.
Mol Ther Oncolytics. 2022 Jun 25;26:175-188.
SUM190
10 μM
4 days
Cardamonin inhibited the formation of mammosphere-like structures induced by poly(I:C) and suppressed the nuclear translocation of NF-κB and β-catenin.
Cell Death Differ. 2015 Feb;22(2):298-310.
SUM190
10 μM
4 days
Cardamonin inhibited the increase in the CD44high/CD24−/low cell subpopulation induced by poly(I:C).
Cell Death Differ. 2015 Feb;22(2):298-310.
SUM190
10 μM
4 days
Cardamonin inhibited the expression of Oct4, c-Myc, and CD44 induced by poly(I:C).
Cell Death Differ. 2015 Feb;22(2):298-310.
Cardamonin/豆蔻明 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
ICR mice
Acute pain models
Intraperitoneal injection
0.3, 1, 3, 10 mg/kg
Single administration
To investigate the antinociceptive effects of Cardamonin in acute pain models and its possible mechanisms. The results showed that Cardamonin significantly inhibited acetic acid-induced abdominal writhing and dose-dependently suppressed pain responses induced by PMA and glutamate.
Molecules. 2020 Nov 18;25(22):5385
SCID mice
SUDHL-4 cell xenograft model
Intragastric administration
15 mg/kg
Once per day for 30 days
To evaluate the in vivo anti-tumor effect of Cardamonin on RRAGC-mutant lymphoma, results showed that Cardamonin significantly inhibited tumor growth in RagCT90N-mutant SUDHL-4 cells.
BMC Complement Med Ther. 2023 Sep 26;23(1):336
Nude mice
MDA-MB-231 xenograft model
Intraperitoneal injection
3 mg/kg
Once daily for four weeks
Cardamonin significantly inhibited tumor growth in the MDA-MB-231 xenograft model
J Exp Clin Cancer Res. 2019 Aug 27;38(1):377.
C57Bl/6 mice
DSS-induced colitis model
Oral
10 mg/kg, 50 mg/kg
Daily for 12 days
Cardamonin attenuated DSS-induced colitis and reduced the accumulation of inflammatory cells in the colon.
Biomolecules. 2021 Apr 29;11(5):661.
BALB/c nude mice
Xenograft model of SKOV3 cells or SKOV3 cells mixed with M2 macrophages
Intraperitoneal injection
5 mg/kg and 25 mg/kg
Once daily for 15 days
Cardamonin significantly inhibited tumor growth and lowered the expression of CD163 and CD206.
Mol Ther Oncolytics. 2022 Jun 25;26:175-188.
Mice
CCI-induced neuropathic pain model
Intraperitoneal injection
10 mg/kg
Single administration
Cardamonin significantly upregulated 5-HT1A receptor subtype expression in the brainstem and spinal cord, indicating its antineuropathic effect in CCI-induced neuropathic pain mice.
Molecules. 2021 Jun 16;26(12):3677
Nude mice
SUM190 xenograft model
Intraperitoneal injection
30 mg/kg
Every other day for 20 days
Cardamonin almost completely abolished the increase in CSCs induced by poly(I:C).
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