There will be a HazMat fee per item when shipping a dangerous goods. The HazMat fee will be charged to your UPS/DHL/FedEx collect account or added to the invoice unless the package is shipped via Ground service. Ship by air in Excepted Quantity (each bottle), which is up to 1g/1mL for class 6.1 packing group I or II, and up to 25g/25ml for all other HazMat items.
Type
HazMat fee for 500 gram (Estimated)
Excepted Quantity
USD 0.00
Limited Quantity
USD 15-60
Inaccessible (Haz class 6.1), Domestic
USD 80+
Inaccessible (Haz class 6.1), International
USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic
USD 100+
Accessible (Haz class 3, 4, 5 or 8), International
Bufalin is an active component isolated from Chan Su, acts as a potent Na+/K+-ATPase inhibitor, binds to the subunit α1, α2 and α3, with Kd of 42.5, 45 and 40 nM, respectively[3]. Bufalin caused significant cytotoxicity in NCI-H460 cells at a concentration as low as 1 μM. DNA condensation was observed in bufalin-treated cells in a dose-dependent manner. Bufalin injected intraperitoneally in a dose-dependent manner reduced tumor size in BALB/C nu/nu mice implanted with NCI-H460 cells. Bufalin injection did not produce significant drug-related toxicity in experimental animals except at a high dose (0.4 mg/kg)[4]. Bufalin-induced disruption of the SRC-1 (steroid receptor coactivator) isoform/ERβ (estrogen receptor) axis might induce apoptosis, pyroptosis and ERS (endoplasmic reticulum-stress) signaling in endometriotic lesions, causing the suppression of endometriosis[5]. Bufalin inhibits cell proliferation and migration of HCC (hepatocellular carcinoma) cells via APOBEC3F induced intestinal immune network for IgA production signaling pathway[6].
Bufalin/蟾毒灵 细胞实验
Cell Line
Concentration
Treated Time
Description
References
SK-N-BE(2)
90 nM
72 hours
Inhibits cell proliferation and migration
Int J Mol Med. 2020 Dec;46(6):2137-2149.
SH-SY5Y
30 nM
72 hours
Inhibits cell proliferation and migration
Int J Mol Med. 2020 Dec;46(6):2137-2149.
Vero cells
0.0625 µM, 0.125 µM, 0.25 µM, 0.5 µM, 1 µM
24 hours
To evaluate the inhibitory effect of Bufalin on HSV-1 replication, results showed Bufalin significantly inhibited HSV-1 replication.
Int J Mol Sci. 2023 Sep 23;24(19):14479.
PK-15 cells
0.015 µM, 0.03 µM, 0.06 µM, 0.12 µM
24 hours
To evaluate the inhibitory effect of Bufalin on PRV replication, results showed Bufalin significantly inhibited PRV replication.
Int J Mol Sci. 2023 Sep 23;24(19):14479.
Human umbilical vein endothelial cells (HUVECs)
10 nM
24 hours
To evaluate the effect of Bufalin on TME-mediated angiogenesis, results showed that Bufalin significantly inhibited HUVEC tube formation, migration, and adhesion induced by TME cells.
J Transl Med. 2021 Sep 8;19(1):383.
L02
0, 20, 40, 80, 160, 320 nM
24, 48, 72 hours
Bufalin had no significant inhibitory effect on normal hepatocyte L02 cells.
J Transl Med. 2023 Dec 11;21(1):900.
RBE
0, 20, 40, 80, 160, 320 nM
24, 48, 72 hours
Bufalin significantly inhibited the proliferation and migration of RBE cells in a dose- and time-dependent manner.
J Transl Med. 2023 Dec 11;21(1):900.
QBC-939
0, 20, 40, 80, 160, 320 nM
24, 48, 72 hours
Bufalin significantly inhibited the proliferation and migration of QBC-939 cells in a dose- and time-dependent manner.
J Transl Med. 2023 Dec 11;21(1):900.
HCCC-9810
0, 20, 40, 80, 160, 320 nM
24, 48, 72 hours
Bufalin significantly inhibited the proliferation and migration of HCCC-9810 cells in a dose- and time-dependent manner.
J Transl Med. 2023 Dec 11;21(1):900.
U266R
24 nM
48 hours
Bufalin alone increased p-AKT levels in U266R cells, and combination with MK2206 enhanced apoptosis induction.
Cell Death Dis. 2017 May 11;8(5):e2776.
H929R
24 nM
48 hours
Bufalin alone increased p-AKT levels in H929R and U266R cells, and combination with MK2206 enhanced apoptosis induction.
Cell Death Dis. 2017 May 11;8(5):e2776.
U266
12 nM
48 hours
Bufalin alone moderately induced apoptosis in U266 cells, accompanied by increased p-AKT levels.
Cell Death Dis. 2017 May 11;8(5):e2776.
NCI-H929
12 nM
48 hours
Bufalin alone moderately induced apoptosis in H929 and U266 cells, accompanied by increased p-AKT levels.
Cell Death Dis. 2017 May 11;8(5):e2776.
HepG2 cells
5, 10, 20 or 50 nM
48 hours
To evaluate the effects of Bufalin on proliferation and apoptosis of HepG2 cells. Bufalin at higher concentrations could inhibit proliferation and induce apoptosis of HepG2 cells.
Oncoimmunology. 2018 Feb 8;7(5):e1426434.
MHCC97 H cells
5, 10, 20 or 50 nM
48 hours
To evaluate the effects of Bufalin on proliferation and apoptosis of HCC cells. Bufalin (higher than 10 nM) could inhibit cell proliferation and induce apoptosis of HCC cells.
Oncoimmunology. 2018 Feb 8;7(5):e1426434.
CEF cells
0.0625 µM, 0.125 µM, 0.25 µM, 0.5 µM, 1 µM
48 hours
To evaluate the inhibitory effect of Bufalin on MDV replication, results showed Bufalin significantly inhibited MDV replication.
Int J Mol Sci. 2023 Sep 23;24(19):14479.
Recombinant human CYP3A4
0, 0.1, 0.5, 1, 2.5, 5, 7.5, 10, 15, 20, 30 µM
5 minutes
To determine the inhibitory effect of Bufalin on CYP3A4 activity, the IC50 value was 14.52 μmol/L.
Acta Pharmacol Sin. 2009 May;30(5):646-52.
Bufalin/蟾毒灵 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
BALB/c mice
PRV infection model
Intraperitoneal injection
0.5 mg/kg
Every 36 hours until all mice in the PRV-infected group had died
To evaluate the therapeutic effect of Bufalin on PRV infection, results showed Bufalin significantly decreased the viral load in multiple tissues and increased the survival rate of mice.
Int J Mol Sci. 2023 Sep 23;24(19):14479.
Balb/c nu/nu mice
HCC xenograft model
Intraperitoneal injection
0.5, 1 or 2 mg/kg
Once a week (poly (I:C)) or five days a week (bufalin) for six weeks
To evaluate the effects of Bufalin on lung metastasis in HCC xenograft model. Bufalin (0.5 mg/kg) could inhibit poly (I:C)-enhanced lung metastasis of HCC cells.
Oncoimmunology. 2018 Feb 8;7(5):e1426434.
BALB/c nu/nu mice
MOPC315 xenograft model
Intraperitoneal injection
1 mg/kg
Once daily for 10 days
Combination treatment significantly inhibited MM tumor growth without significant side effects.
Subcutaneous xenograft tumor model and liver metastasis model
Intraperitoneal injection
1 mg/kg
Once every other day for 21 days (flank) or 14 days (spleen)
To evaluate the antiangiogenic activity of Bufalin in vivo, results showed that Bufalin significantly inhibited tumor growth and liver metastasis, reducing the number of tumor blood vessels and STAT3 phosphorylation in vascular endothelial cells.
J Transl Med. 2021 Sep 8;19(1):383.
SCID/NOD mice
MM xenograft model
Intraperitoneal injection
1 mg/kg
Twice weekly
AHSA1 overexpression promotes MM cell growth and BTZ resistance
J Exp Clin Cancer Res. 2022 Jan 6;41(1):11
Wistar rats
Healthy male Wistar rats
Oral (ig) and intravenous (iv) injection
10 mg/kg
Single dose
To evaluate the effect of Bufalin on CYP3A4 activity, results showed that Bufalin significantly increased AUC0–t and t1/2, and decreased CL and the formation of 1-hydroxy-midazolam.
Acta Pharmacol Sin. 2009 May;30(5):646-52.
C57BL/6 mice
Orthotopic HCC model
Intraperitoneal injection
10 μg/kg
Every other day for 3 weeks
To investigate the inhibitory effect of bufalin on HCC in immunocompetent mice, results showed that bufalin promoted macrophage polarization toward M1 phenotype, activated antitumor T cell immune response, and thereby suppressed HCC growth.
J Immunother Cancer. 2022 May;10(5):e004297
BALB/c nude mice
4T1 breast cancer model
Intravenous injection
2 mg/kg
Every 2 days for 3 weeks
Evaluate the in vivo antitumor efficacy of FA-MOF/Buf NPs, showing significant tumor growth inhibition and reduced side effects.
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