货号:A2926272
同义名:
重组生物素化人Latent TGF-beta 3蛋白(N-8His-Avi, HEK293)
/ Biotinylated Human Latent TGF-beta 3 (N-8His-Avi, HEK293); Transforming growth factor beta-3 proprotein
Biotinylated Human Latent TGF-beta 3 (N-8His-Avi, HEK293) 是人源蛋白,在HEK293细胞中表达,带有N端8His-Avi标签。该蛋白为转化生长因子β家族成员TGF-β3,以潜伏形式存在,可通过整合素作用释放活性形式,高亲和力结合TGF-β RII受体,进而激活Smad信号通路,调控细胞生长、分化、迁移及凋亡等关键生物学过程。


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| 描述 | Transforming growth factor beta (TGFβ) is a multifunctional cytokine that regulates cell growth, differentiation, adhesion, migration and death dependent on cell type, developmental stage, or tissue conditions. There are three isoforms of TGFβ (TGFβ-1, -2 and -3). TGF-beta-3 is released from LAP by integrins: integrin-binding results in distortion of the LAP chain and subsequent release of the active TGF-beta-3. TGF-beta 3 binds with high affinity to TGF-beta RII, a type II serine/threonine kinase receptor. This receptor then phosphorylates and activates type I serine/threonine kinase receptors, TGF-beta RI or ALK-1, to modulate transcription through Smad phosphorylation. |
| 蛋白种属 | Human |
| 表达系统 | Human cells |
| 标签 | N-8His-Avi |
| 蛋白表达区间 | Leu30-Ser390 (Cys33Ser) |
| 蛋白编号 | P10600 |
| 内毒素 | < 1 EU/µg as determined by LAL test. |
| 剂型 | 干粉 |
| 剂型描述 | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存,建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释,如果是做无血清培养或者动物体内实验时,细胞因子中不能含有BSA、FBS 和 HAS,可以使用海藻糖作为载体,然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子,并避免反复冻融;稀释分装浓度不低于 10μg/mL,分装体积不低于10μL。 分装后的蛋白在≤-20°C 下可稳定保存 3 个月。 |
| CAS号 | N/A |
| 分子量 | 12.7&35.3 KDa |
| SMILES Code | NONE |
| 别名 | 重组生物素化人Latent TGF-beta 3蛋白(N-8His-Avi, HEK293) ;Biotinylated Human Latent TGF-beta 3 (N-8His-Avi, HEK293); Transforming growth factor beta-3 proprotein; TGF-beta-3; TGFβ3; TGFB3 |
| 运输 | 蓝冰 |
| 存储条件 |
In solvent -20°C: 3-6个月 -80°C: 12个月 Pure form Sealed in dry, store in freezer, under -20°C |
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