Astragaloside I (As-I), one of the main active ingredients in Astragalus membranaceus, has osteogenic properties. As-I stimulated the expression of β-catenin and Runx2 in MC3T3-E1 cells. Furthermore, As-I also increased BMP-2, BGP and OPG/RANKL expression, which are also activated by Wnt/β-catenin signaling pathway. Astragaloside I stimulates osteoblast differentiation through the Wnt/β-catenin signaling pathway. Astragaloside I (10-40 μM) upregulates the express of β-catenin, Runx2, BGP and OPG, RANKL (osteogenesis marker genes) in MC3T3-E1 cells[2]. Astragaloside I (A), levistilide A (L) and calycosin (C) produced synergistic proliferation inhibition on LX-2 cells and TGF-β1-activated LX-2 cells. Administration of the ALC formula markedly decreased collagen deposition, hydroxyproline (Hyp) content and α-SMA expression levels in the liver tissues compared to the model mice. Astragaloside I, levistilide A and calycosin may be the 3 main bioactive components in DBT(Danggui Buxue Tang); their combination exerts anti-liver fibrosis effects in vitro and in vivo[3].
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