There will be a HazMat fee per item when shipping a dangerous goods. The HazMat fee will be charged to your UPS/DHL/FedEx collect account or added to the invoice unless the package is shipped via Ground service. Ship by air in Excepted Quantity (each bottle), which is up to 1g/1mL for class 6.1 packing group I or II, and up to 25g/25ml for all other HazMat items.
Type
HazMat fee for 500 gram (Estimated)
Excepted Quantity
USD 0.00
Limited Quantity
USD 15-60
Inaccessible (Haz class 6.1), Domestic
USD 80+
Inaccessible (Haz class 6.1), International
USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic
USD 100+
Accessible (Haz class 3, 4, 5 or 8), International
AY 9944 is a specific cholesterol biosynthesis inhibitor that inhibits 7-dehydrocholesterol Δ7 reductase (DHCR7) with an IC50 of 13 nM. AY 9944 causes hypocholesterolaemia and accumulation of 7DHC. At high doses, AY 9944 also inhibits the sterol Δ7-Δ8 isomerase in cultured embryos, leading to the accumulation of cholesterol-8-en-3β-ol[1][2][3].AY 9944 affects the type of free sterols in keratinocytes at a concentration of 1 µg/ml for 15 h. AY 9944 inhibits the synthesis of cholesterol and desmosterol, but increases the synthesis of 7-dehydrocholesterol and oleanol[2].
AY 9944 细胞实验
Cell Line
Concentration
Treated Time
Description
References
Human hepatocellular carcinoma Huh-7 cells
10-100 nM
24 hours
Inhibition of DHCR7 activity increases 7-DHC levels, thereby suppressing ferroptosis
Nat Commun. 2024 Mar 12;15(1):2195.
AY 9944 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Mice
Hepatic ischemia-reperfusion injury (IRI) model
Intraperitoneal injection
25 mg/kg
Single dose
Inhibition of hepatic ferroptosis and reduction of liver injury
Nat Commun. 2024 Mar 12;15(1):2195.
Sprague-Dawley rats
Pharmacological rat model of Smith-Lemli-Opitz Syndrome (SLOS)
Subcutaneous injection
30 mg/kg
Starting on postnatal day one, 3 times per week, up to 3 months of age
To mimic sterol synthesis defects in SLOS by inhibiting DHCR7 enzyme activity, observing pathological changes in retinal pigment epithelium (RPE) and neural retina. Results showed markedly increased RPE apical autofluorescence correlating with elevated A2E and other bisretinoid levels.
Autophagy. 2018;14(10):1796-1817
C57BL/6 mice
Kidney ischemia-reperfusion injury (IRI) model
Intraperitoneal injection
25 mg/kg
Once daily for 4 times
Increasing endogenous 7-DHC levels by inhibiting DHCR7 significantly protected the kidney from IRI, reducing blood urea nitrogen (BUN) and serum creatinine (SCr) levels.
Nature. 2024 Feb;626(7998):411-418
AY 9944 动物研究
Animal study
At a dose of 7.5 mg/kg, administered every 6 days from postnatal day 2 to postnatal day 20, i.h., AY 9944 reduced brain cholesterol in Long-Evans hooded rats until 400 days after cessation of treatment. Brain cholesterol was reduced and 7-dehydrocholesterol was elevated in all brain regions of the rats, and AY 9944 had more severe effects on changes in plasma and liver steroids in female rats[3].
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